The βII Isotype of Tubulin Is Present in the Cell Nuclei of a Variety of Cancers

I. Tien Yeh, Richard F. Ludueña

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Tubulin, the subunit protein of microtubules, has generally been thought to be exclusively a cytoplasmic protein in higher eukaryotes. We have previously shown that cultured rat kidney mesangial cells contain the βII isotype of tubulin in their nuclei in the form of an αβII dimer [Walss et al., 1999: Cell Motil. Cytoskeleton 42:274-284, 1999]. More recently, we examined a variety of cancerous and non-cancerous cell lines and found βII in the nuclei of all of the former and only a few of the latter (Walss-Bass et al., 2002: Cell Tissue Res. 308:215-223]. In order to determine if βII-tubulin occurs in the nuclei of actual cancers as well as in cancer cell lines, we used the immunoperoxidase method to look for nuclear βII in a variety of tumors excised from 201 patients. We found that 75% of these tumors contain βII in their nuclei. Distribution of nuclear β II was highly dependent on the type of cancer, with 100% of the colon and prostate cancers, but only 19% of the skin tumors, having nuclear βII. Nuclear βII was particularly marked in tumors of epithelial origin, of which 83% showed nuclear βII, in contrast to 54% in tumors of non-epithelial origin. In many cases, βII staining occurred very strongly in the nuclei and not in the cytoplasm; in other cases, βII was present in both. In many cases, particularly metastases, otherwise normal cells adjacent to the tumor also showed nuclear βII, suggesting that cancer cells may influence nearby cells to synthesize βII and localize it to their nuclei. Our results have implications for the diagnosis, biology, and chemotherapy of cancer.

Original languageEnglish (US)
Pages (from-to)96-106
Number of pages11
JournalCell Motility and the Cytoskeleton
Volume57
Issue number2
DOIs
StatePublished - Feb 2004

Fingerprint

Tubulin
Cell Nucleus
Neoplasms
Microtubule Proteins
Cell Line
Mesangial Cells
Protein Subunits
Eukaryota
Cytoskeleton
Colonic Neoplasms
Prostatic Neoplasms
Cytoplasm
Staining and Labeling
Neoplasm Metastasis
Drug Therapy
Skin

Keywords

  • β
  • Cancer
  • Cell nucleus
  • Isotypes
  • Metastasis
  • Tubulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

The βII Isotype of Tubulin Is Present in the Cell Nuclei of a Variety of Cancers. / Yeh, I. Tien; Ludueña, Richard F.

In: Cell Motility and the Cytoskeleton, Vol. 57, No. 2, 02.2004, p. 96-106.

Research output: Contribution to journalArticle

Yeh, I. Tien ; Ludueña, Richard F. / The βII Isotype of Tubulin Is Present in the Cell Nuclei of a Variety of Cancers. In: Cell Motility and the Cytoskeleton. 2004 ; Vol. 57, No. 2. pp. 96-106.
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