Abstract
The differences among the vertebrate b isotypes of tubulin are highly conserved in evolution, suggesting that they have functional significance. To address this, we have used differentiating neuroblastoma cells as a model system. These cells express the bI, bII, and bIII isotypes. Although there is no difference prior to differentiation, a striking difference is seen after differentiation. Both bI and bIII occur in cell bodies and neurites, while bII occurs mostly in neurites. Knocking down bI causes a large decrease in cell viability while silencing bII and bIII does not. Knocking down bII causes a large decrease in neurite outgrowth without affecting viability. Knocking down bIII has little effect on neurite outgrowth and only decreases viability if cells are treated with glutamate and glycine, a combination known to generate free radicals and reactive oxygen species. It appears, therefore, that bI is required for cell viability, bII for neurite outgrowth and bIII for protection against free radicals and reactive oxygen species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 431-441 |
| Number of pages | 11 |
| Journal | Cytoskeleton |
| Volume | 67 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2010 |
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Keywords
- βI-tubulin
- βII-tubulin
- βIII-tubulin
- Microtubules
- Neuroblastoma
- Tubulin
ASJC Scopus subject areas
- Cell Biology
- Structural Biology
Cite this
The β isotypes of tubulin in neuronal differentiation. / Guo, Jiayan; Walss-Bass, Consuelo; Ludueña, Richard F.
In: Cytoskeleton, Vol. 67, No. 7, 07.2010, p. 431-441.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The β isotypes of tubulin in neuronal differentiation
AU - Guo, Jiayan
AU - Walss-Bass, Consuelo
AU - Ludueña, Richard F.
PY - 2010/7
Y1 - 2010/7
N2 - The differences among the vertebrate b isotypes of tubulin are highly conserved in evolution, suggesting that they have functional significance. To address this, we have used differentiating neuroblastoma cells as a model system. These cells express the bI, bII, and bIII isotypes. Although there is no difference prior to differentiation, a striking difference is seen after differentiation. Both bI and bIII occur in cell bodies and neurites, while bII occurs mostly in neurites. Knocking down bI causes a large decrease in cell viability while silencing bII and bIII does not. Knocking down bII causes a large decrease in neurite outgrowth without affecting viability. Knocking down bIII has little effect on neurite outgrowth and only decreases viability if cells are treated with glutamate and glycine, a combination known to generate free radicals and reactive oxygen species. It appears, therefore, that bI is required for cell viability, bII for neurite outgrowth and bIII for protection against free radicals and reactive oxygen species.
AB - The differences among the vertebrate b isotypes of tubulin are highly conserved in evolution, suggesting that they have functional significance. To address this, we have used differentiating neuroblastoma cells as a model system. These cells express the bI, bII, and bIII isotypes. Although there is no difference prior to differentiation, a striking difference is seen after differentiation. Both bI and bIII occur in cell bodies and neurites, while bII occurs mostly in neurites. Knocking down bI causes a large decrease in cell viability while silencing bII and bIII does not. Knocking down bII causes a large decrease in neurite outgrowth without affecting viability. Knocking down bIII has little effect on neurite outgrowth and only decreases viability if cells are treated with glutamate and glycine, a combination known to generate free radicals and reactive oxygen species. It appears, therefore, that bI is required for cell viability, bII for neurite outgrowth and bIII for protection against free radicals and reactive oxygen species.
KW - βI-tubulin
KW - βII-tubulin
KW - βIII-tubulin
KW - Microtubules
KW - Neuroblastoma
KW - Tubulin
UR - http://www.scopus.com/inward/record.url?scp=77955899519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955899519&partnerID=8YFLogxK
U2 - 10.1002/cm.20455
DO - 10.1002/cm.20455
M3 - Article
C2 - 20506160
AN - SCOPUS:77955899519
VL - 67
SP - 431
EP - 441
JO - Cytoskeleton
JF - Cytoskeleton
SN - 1949-3584
IS - 7
ER -