TGF-β1 null mutation leads to CD154 upregulated expression in affected tissues

Toru Nakabayashi; Ken Mei Sakata; Atsuko Sakata; Liping Kong; C. Allen Lau; John J. Letterio; Norma Vela-Roch; Norman Talal; Howard Dang

doi:10.1023/A:1007158219974

TGF-β1 null mutation leads to CD154 upregulated expression in affected tissues

Toru Nakabayashi, Ken Mei Sakata, Atsuko Sakata, Liping Kong, C. Allen Lau, John J. Letterio, Norma Vela-Roch, Norman Talal, Howard Dang

Developmental Dentistry

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The TGF-β1(-/-) mouse is a murine model for systemic autoimmune disease. The aim of this study is to elucidate the immunological mechanism that leads to multifocal tissue inflammation and autoantibody production in TGF-β1(-/-) mice. Heart, lung, liver, and salivary gland from TGF-β1(-/-) were assessed for CD154 expression by RT-PCR and immunohistochemistry. Compared to wild-type littermates, CD154 expression was elevated in all tissues studied. Furthermore, IL-12 mRNA was expressed in the salivary gland and heart of TGF-β1(-/-) mice and not in wild-type littermates. This suggests that the CD154 pathway is activated in these tissues. This shows that TGF-β1 regulates CD154 expression leading to spontaneous IL-12 production and autoimmunity.

Original language	English (US)
Pages (from-to)	69-73
Number of pages	5
Journal	Inflammation
Volume	25
Issue number	2
DOIs	https://doi.org/10.1023/A:1007158219974
State	Published - 2001

Keywords

CD154
Interleukin (IL-12)
Tissue inflammation
Transforming growth factor (TGF-β1)

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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title = "TGF-β1 null mutation leads to CD154 upregulated expression in affected tissues",

abstract = "The TGF-β1(-/-) mouse is a murine model for systemic autoimmune disease. The aim of this study is to elucidate the immunological mechanism that leads to multifocal tissue inflammation and autoantibody production in TGF-β1(-/-) mice. Heart, lung, liver, and salivary gland from TGF-β1(-/-) were assessed for CD154 expression by RT-PCR and immunohistochemistry. Compared to wild-type littermates, CD154 expression was elevated in all tissues studied. Furthermore, IL-12 mRNA was expressed in the salivary gland and heart of TGF-β1(-/-) mice and not in wild-type littermates. This suggests that the CD154 pathway is activated in these tissues. This shows that TGF-β1 regulates CD154 expression leading to spontaneous IL-12 production and autoimmunity.",

keywords = "CD154, Interleukin (IL-12), Tissue inflammation, Transforming growth factor (TGF-β1)",

author = "Toru Nakabayashi and Sakata, {Ken Mei} and Atsuko Sakata and Liping Kong and Lau, {C. Allen} and Letterio, {John J.} and Norma Vela-Roch and Norman Talal and Howard Dang",

year = "2001",

doi = "10.1023/A:1007158219974",

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T1 - TGF-β1 null mutation leads to CD154 upregulated expression in affected tissues

AU - Nakabayashi, Toru

AU - Sakata, Ken Mei

AU - Sakata, Atsuko

AU - Kong, Liping

AU - Lau, C. Allen

AU - Letterio, John J.

AU - Vela-Roch, Norma

AU - Talal, Norman

AU - Dang, Howard

PY - 2001

Y1 - 2001

N2 - The TGF-β1(-/-) mouse is a murine model for systemic autoimmune disease. The aim of this study is to elucidate the immunological mechanism that leads to multifocal tissue inflammation and autoantibody production in TGF-β1(-/-) mice. Heart, lung, liver, and salivary gland from TGF-β1(-/-) were assessed for CD154 expression by RT-PCR and immunohistochemistry. Compared to wild-type littermates, CD154 expression was elevated in all tissues studied. Furthermore, IL-12 mRNA was expressed in the salivary gland and heart of TGF-β1(-/-) mice and not in wild-type littermates. This suggests that the CD154 pathway is activated in these tissues. This shows that TGF-β1 regulates CD154 expression leading to spontaneous IL-12 production and autoimmunity.

AB - The TGF-β1(-/-) mouse is a murine model for systemic autoimmune disease. The aim of this study is to elucidate the immunological mechanism that leads to multifocal tissue inflammation and autoantibody production in TGF-β1(-/-) mice. Heart, lung, liver, and salivary gland from TGF-β1(-/-) were assessed for CD154 expression by RT-PCR and immunohistochemistry. Compared to wild-type littermates, CD154 expression was elevated in all tissues studied. Furthermore, IL-12 mRNA was expressed in the salivary gland and heart of TGF-β1(-/-) mice and not in wild-type littermates. This suggests that the CD154 pathway is activated in these tissues. This shows that TGF-β1 regulates CD154 expression leading to spontaneous IL-12 production and autoimmunity.

KW - CD154

KW - Interleukin (IL-12)

KW - Tissue inflammation

KW - Transforming growth factor (TGF-β1)

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