Abstract
Transforming growth factor β-1 (TGFβ-1) is a multifunctional growth factor that is expressed in numerous cell types. It has been shown to induce secretion of dentin extracellular matrix components associated with primary dentinogenesis and to play a role in tertiary or reparative dentinogenesis. In this study, we investigated the potential transcriptional regulation by TGFβ-1 of two dentin matrix proteins: dentin matrix protein 1 (DMP-1), and dentin sialophosphoprotein (DSPP). In vitro promoter studies were performed using plasmid constructs containing mouse DMP-1 and DSPP promoter sequences fused to the luciferase reporter gene. Constructs were transiently transfected in the mouse odontoblast cell line M06-G3 and cultured in the presence or absence of TGFβ-1. The integrity of the TGFβ-1 signaling pathway was investigated in the M06-G3 cells by identifying known key effectors of TGFβ-1 signal transduction. Transient transfection studies demonstrate for the first time that TGFβ-1 downregulates both DMP-1 and DSPP genes. Our findings indicate that the TGFβ-1 type I receptor ALK5 is expressed by odontoblasts as well as the signal transduction proteins Smad2, Smad3, and Smad4. These results suggest that TGFβ-1 regulates two key dentin proteins involved in matrix mineralization most likely mediated through the type I ALK5 receptor and transduced by Smads 2, 3, and 4.
Original language | English (US) |
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Pages (from-to) | 354-358 |
Number of pages | 5 |
Journal | Connective Tissue Research |
Volume | 43 |
Issue number | 2-3 |
DOIs | |
State | Published - 2002 |
Keywords
- ALK5
- Dentin matrix protein 1
- Dentin sialophosphoprotein
- Smads
- TGFBR1
- TGFβ-1
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry
- Rheumatology
- Cell Biology
- Orthopedics and Sports Medicine