TET2 mutations and their clinical correlates in polycythemia vera, essential thrombocythemia and myelofibrosis

A. Tefferi, A. Pardanani, K. H. Lim, O. Abdel-Wahab, T. L. Lasho, J. Patel, N. Gangat, C. M. Finke, S. Schwager, A. Mullally, C. Y. Li, C. A. Hanson, R. Mesa, O. Bernard, F. Delhommeau, W. Vainchenker, D. G. Gilliland, R. L. Levine

Research output: Contribution to journalArticlepeer-review

309 Scopus citations

Abstract

High-throughput DNA sequence analysis was used to screen for TET2 mutations in bone marrow-derived DNA from 239 patients with BCR-ABL-negative myeloproliferative neoplasms (MPNs). Thirty-two mutations (19 frameshift, 10 nonsense, 3 missense; mostly involving exons 4 and 12) were identified for an overall mutational frequency of ∼13%. Specific diagnoses included polycythemia vera (PV; n = 89), essential thrombocythemia (ET; n = 57), primary myelofibrosis (PMF; n = 60), post-PV MF (n = 14), post-ET MF (n = 7) and blast phase PV/ET/MF (n = 12); the corresponding mutational frequencies were ∼16, 5, 17, 14, 14 and 17% (P=0.50). Mutant TET2 was detected in ∼17 and ∼7% of JAK2V617F-positive and -negative cases, respectively (P=0.04). However, this apparent clustering of the two mutations was accounted for by an independent association between mutant TET2 and advanced age; mutational frequency was ∼23% in patients ≥60 years old versus ∼4% in younger patients (P < 0.0001). The presence of mutant TET2 did not affect survival, leukemic transformation or thrombosis in either PV or PMF; a correlation with hemoglobin <10 g per 100ml in PMF was noted (P = 0.05). We conclude that TET2 mutations occur in both JAK2V617F-positive and -negative MPN, are more prevalent in older patients, display similar frequencies across MPN subcategories and disease stages, and hold limited prognostic relevance.

Original languageEnglish (US)
Pages (from-to)905-911
Number of pages7
JournalLeukemia
Volume23
Issue number5
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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