Testis and somatic Xrcc-1 DNA repair gene expression

Christi A. Walter, Jianwei Lu, Mukesh Bhakta, Zi Qiang Zhou, Larry H. Thompson, John R. McCarrey

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The human XRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose of this study was to characterize the expression of Xrcc-1 to determine if there is tissue-specific expression and to provide a baseline of information for future studies that may involve altering Xrcc-1 expression in mice. Normal young adult male testis and enriched populations of pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissue, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to male germ cells, which is a striking contrast to the levels of expression obtained in somatic tissues from the mouse. The relatively high levels of expression identified in male germ cells indicate Xrcc-1 may have an important role in male germ cell physiology.

Original languageEnglish (US)
Pages (from-to)451-461
Number of pages11
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number6
DOIs
StatePublished - Nov 1 1994

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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