Abstract
A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells. Embryos between E10.5-E18.5 days were analyzed for expression of the transgene after staining for β-galactosidase activity. Transgene expression was restricted to the skeletal muscle lineages reflecting a similar temporal and spatial pattern previously demonstrated for the endogenous myogenin gene. Additionally, a second transgene, MC1tk, showed expression in 87% of the clones when targeted to Hprt. This strategy, called targeted transgenesis, provides control for analyzing promoter sequences and for comparing various transgenes expressed by the same promoter.
Original language | English (US) |
---|---|
Pages (from-to) | 154-163 |
Number of pages | 10 |
Journal | BioTechniques |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - 1999 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)