Abstract
A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells. Embryos between E10.5-E18.5 days were analyzed for expression of the transgene after staining for β-galactosidase activity. Transgene expression was restricted to the skeletal muscle lineages reflecting a similar temporal and spatial pattern previously demonstrated for the endogenous myogenin gene. Additionally, a second transgene, MC1tk, showed expression in 87% of the clones when targeted to Hprt. This strategy, called targeted transgenesis, provides control for analyzing promoter sequences and for comparing various transgenes expressed by the same promoter.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-163 |
| Number of pages | 10 |
| Journal | BioTechniques |
| Volume | 27 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1999 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
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Vivian, J. L., Klein, W. H., & Hasty, P. (1999). Temporal, spatial and tissue-specific expression of a myogenin-lacZ transgene targeted to the Hprt locus in mice. BioTechniques, 27(1), 154-163. https://doi.org/10.2144/99271rr04