Temporal proteomic profiling of Chlamydia trachomatis-infected HeLa-229 human cervical epithelial cells

Grace Min Yi Tan, Hui Jing Lim, Tee Cian Yeow, Elaheh Movahed, Chung Yeng Looi, Rishein Gupta, Bernard P. Arulanandam, Sazaly Abu Bakar, Negar Shafiei Sabet, Li Yen Chang, Won Fen Wong

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Chlamydia trachomatis is the leading causative agent of bacterial sexually transmitted infections worldwide which can lead to female pelvic inflammatory disease and infertility. A greater understanding of host response during chlamydial infection is essential to design intervention technique to reduce the increasing incidence rate of genital chlamydial infection. In this study, we investigated proteome changes in epithelial cells during C. trachomatis infection by using an isobaric tags for relative and absolute quantitation (iTRAQ) labeling technique coupled with a liquid chromatography-tandem mass spectrometry (LC-MS3) analysis. C. trachomatis (serovar D, MOI 1)-infected HeLa-229 human cervical carcinoma epithelial cells (at 2, 4 and 8 h) showed profound modifications of proteome profile which involved 606 host proteins. MGST1, SUGP2 and ATXN10 were among the top in the list of the differentially upregulated protein. Through pathway analysis, we suggested the involvement of eukaryotic initiation factor 2 (eIF2) and mammalian target of rapamycin (mTOR) in host cells upon C. trachomatis infection. Network analysis underscored the participation of DNA repair mechanism during C. trachomatis infection. In summary, intense modifications of proteome profile in C. trachomatis-infected HeLa-229 cells indicate complex host-pathogen interactions at early phase of chlamydial infection.

Original languageEnglish (US)
Pages (from-to)1347-1360
Number of pages14
Issue number9
StatePublished - May 1 2016


  • Cervical epithelial cells
  • Chlamydia trachomatis
  • Mass spectrometry
  • Proteome expression
  • Sexual transmitted infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


Dive into the research topics of 'Temporal proteomic profiling of Chlamydia trachomatis-infected HeLa-229 human cervical epithelial cells'. Together they form a unique fingerprint.

Cite this