Temperature effects on solutions of vinblastine-induced polymers assembled from brine shrimp (Anemia) tubulin

S. A. MacKinlay, R. F. Ludueña, T. H. MacRae

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purified Artemia tubulin in the presence of neural microtubule-associated proteins and vinblastine, or with vinblastine alone, forms extensive coils. Reduction in temperature of a coil-containing solution to 4°C causes an increase in turbidity, which returns to previous levels once the solution is warmed. Examination of negatively stained samples indicates that the turbidity fluctuations are not accompanied by a pronounced change in coil structure nor by increased polymer formation. Bovine neural tubulin responds in the same way as Artemia tubulin to vinblastine and temperature. An interesting novel response to vinblastine, shared by tubulins from phylogenetically distinct organisms, is illustrated by our results.

Original languageEnglish (US)
Pages (from-to)301-305
Number of pages5
JournalFEBS Letters
Volume203
Issue number2
DOIs
StatePublished - Jul 28 1986

Fingerprint

Artemia
Vinblastine
Tubulin
Thermal effects
Anemia
Polymers
Temperature
Turbidity
Microtubule-Associated Proteins
brine

Keywords

  • (Artemia)
  • Microtubule-associated protein
  • Temperature effect
  • Tubulin
  • Vinblastine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Temperature effects on solutions of vinblastine-induced polymers assembled from brine shrimp (Anemia) tubulin. / MacKinlay, S. A.; Ludueña, R. F.; MacRae, T. H.

In: FEBS Letters, Vol. 203, No. 2, 28.07.1986, p. 301-305.

Research output: Contribution to journalArticle

MacKinlay, S. A. ; Ludueña, R. F. ; MacRae, T. H. / Temperature effects on solutions of vinblastine-induced polymers assembled from brine shrimp (Anemia) tubulin. In: FEBS Letters. 1986 ; Vol. 203, No. 2. pp. 301-305.
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