Abstract
We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7- kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.
Original language | English (US) |
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Pages (from-to) | 2404-2410 |
Number of pages | 7 |
Journal | Molecular and cellular biology |
Volume | 14 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology