Targeting frequency for deletion vectors in embryonic stem cells

Hongbing Zhang, Paul Hasty, Allan Bradley

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

We analyzed the gene targeting frequencies and recombination products generated by a series of replacement deletion vectors which target the hprt (hypoxanthine phosphoribosyltransferase) locus in mouse embryonic stem cells. We found that the targeting frequency of a 19.2-kb deletion was comparable to that of a 3-kb deletion or a conventional replacement event in which a 1.7- kb fragment was inserted into the locus. We also observed different integration patterns for these deletion vectors. A result of this finding is that a wide range of genomic deletions in embryonic stem cells is feasible.

Original languageEnglish (US)
Pages (from-to)2404-2410
Number of pages7
JournalMolecular and cellular biology
Volume14
Issue number4
DOIs
StatePublished - Apr 1994

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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