T4 AsiA blocks DNA recognition by remodeling σ70 region 4

Lester J. Lambert, Yufeng Wei, Virgil Schirf, Borries Demeler, Milton H. Werner

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Bacteriophage T4 AsiA is a versatile transcription factor capable of inhibiting host gene expression as an 'anti-σ' factor while simultaneously promoting gene-specific expression of T4 middle genes in conjunction with T4 MotA. To accomplish this task, AsiA engages conserved region 4 of Eschericia coli σ70, blocking recognition of most host promoters by sequestering the DNA-binding surface at the AsiA/σ70 interface. The three-dimensional structure of an AsiA/region 4 complex reveals that the C-terminal α helix of region 4 is unstructured, while four other helices adopt a completely different conformation relative to the canonical structure of unbound region 4. That AsiA induces, rather than merely stabilizes, this rearrangement can be realized by comparison to the homologous structures of region 4 solved in a variety of contexts, including the structure of Thermotoga maritima σA region 4 described herein. AsiA simultaneously occupies the surface of region 4 that ordinarily contacts core RNA polymerase (RNAP), suggesting that an AsiA-bound σ70 may also undergo conformational changes in the context of the RNAP holoenzyme.

Original languageEnglish (US)
Pages (from-to)2952-2962
Number of pages11
JournalEMBO Journal
Volume23
Issue number15
DOIs
StatePublished - Aug 4 2004

Fingerprint

DNA-Directed RNA Polymerases
Genes
Holoenzymes
Bacteriophages
DNA
Gene expression
Conformations
Transcription Factors
Thermotoga maritima
Gene Expression
Bacteriophage T4

Keywords

  • AsiA
  • NMR
  • RNA polymerase
  • Sigma70
  • Structure

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Lambert, L. J., Wei, Y., Schirf, V., Demeler, B., & Werner, M. H. (2004). T4 AsiA blocks DNA recognition by remodeling σ70 region 4. EMBO Journal, 23(15), 2952-2962. https://doi.org/10.1038/sj.emboj.7600312

T4 AsiA blocks DNA recognition by remodeling σ70 region 4. / Lambert, Lester J.; Wei, Yufeng; Schirf, Virgil; Demeler, Borries; Werner, Milton H.

In: EMBO Journal, Vol. 23, No. 15, 04.08.2004, p. 2952-2962.

Research output: Contribution to journalArticle

Lambert, LJ, Wei, Y, Schirf, V, Demeler, B & Werner, MH 2004, 'T4 AsiA blocks DNA recognition by remodeling σ70 region 4', EMBO Journal, vol. 23, no. 15, pp. 2952-2962. https://doi.org/10.1038/sj.emboj.7600312
Lambert LJ, Wei Y, Schirf V, Demeler B, Werner MH. T4 AsiA blocks DNA recognition by remodeling σ70 region 4. EMBO Journal. 2004 Aug 4;23(15):2952-2962. https://doi.org/10.1038/sj.emboj.7600312
Lambert, Lester J. ; Wei, Yufeng ; Schirf, Virgil ; Demeler, Borries ; Werner, Milton H. / T4 AsiA blocks DNA recognition by remodeling σ70 region 4. In: EMBO Journal. 2004 ; Vol. 23, No. 15. pp. 2952-2962.
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