T2Candida provides rapid and accurate species identification in pediatric cases of Candidemia

Camille L. Hamula, Kenneth Hughes, Brian T. Fisher, Theoklis E. Zaoutis, Ila R. Singh, Aristea Velegraki

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Objectives: The goal of this study is to assess the ability of the T2Candida platform (T2 Biosystems, Lexington, MA) to accurately identify Candida species from pediatric blood specimens with low volumes. Methods: Whole blood from 15 children with candidemia was collected immediately following blood culture draw. The amount of blood required by the system was reduced by pipetting whole blood directly onto the T2Candida cartridge. Specimens were subsequently run on the T2Dx Instrument (T2 Biosystems). Results: The T2Candida panel provided the appropriate result for each specimen compared with blood culture-based species identification and correctly identified 15 positive and nine negative results in 3 to 5 hours. While the time to species identification for blood culture was not reported, the T2Candida results include species data. Conclusions: T2Candida can be used to efficiently diagnose or rule out candidemia using low-volume blood specimens from pediatric patients. This could result in improved time to appropriate antifungal therapy or reduction in unnecessary empirical antifungal therapy.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalAmerican journal of clinical pathology
Volume145
Issue number6
DOIs
StatePublished - Jun 1 2016
Externally publishedYes

Keywords

  • Candida
  • Direct detection
  • T2Candida

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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