Systemic administration of vascular endothelial growth factor monoclonal antibody reduces the growth of papillary thyroid carcinoma in a nude mouse model

Andrew J. Bauer, Aneeta Patel, Richard Terrell, Krishna Doniparthi, Motoyasu Saji, Matthew Ringel, R. Michael Tuttle, Gary L. Francis

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Papillary thyroid carcinomas (PTC) are the most common thyroid cancers in children. Most are successfully treated with surgery and radioactive iodine, but some persist. PTC express high levels of vascular endothelial growth factor (VEGF) and VEGF receptor (Flt-1). PTC with the most intense expression of VEGF have the greatest recurrence risk. We hypothesized that blockade of VEGF would inhibit PTC growth. To test this, we used systemic VEGF monoclonal antibody (VEGF-MAb) to treat PTC xenografts in nude mice. Treated animals (n = 9) received 200 μg VEGF-MAb by daily ip injection for 10 wk, while control animals (n = 9) received vehicle alone. Tumor size was significantly reduced in the treatment group (0.28 ± 0.06 vs 1.05 ± 0.25 g, p = 0.008). VEGF immunostaining was more intense (2.57 ± 0.30 vs 1.75 ± 0.25, p = 0.06) and the number of p53 positive cells was increased (1.66 ± 0.24 vs 0.83 ± 0.3 1, p = 0.048) in treated tumors. Animal weight was similar in both groups (29.1 ± 1.1 vs 27.4 ± 1.1 g, p = 0.30). In conclusion, systemic VEGF-MAb significantly reduced the growth of PTC, suggesting that VEGF-MAb might be useful for treatment of resistant PTC.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalAnnals of Clinical and Laboratory Science
Volume33
Issue number2
StatePublished - Mar 1 2003
Externally publishedYes

Keywords

  • Papillary thyroid carcinoma
  • Thyroid cancer
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology
  • Molecular Biology
  • Hematology
  • Clinical Biochemistry
  • Medical Laboratory Technology

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