Systematic identification of LINE-1 repetitive DNA sequence differences having species specificity between Mus spretus and Mus domesticus

Brad A. Rikke, Lorrie D. Garvin, Stephen C. Hardies

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

LINE-1 is a family of repetitive DNA sequences interspersed among mammalian genes. In the mouse haploid genome there are about 100,000 LINE-1 copies. We asked if the subspecies Mus spretus and Mus domesticus have developed species-specific LINE-1 subfamilies. Sequences from 14 M. spretus LINE-1 elements were obtained and compared to M. domesticus LINE-1 sequences. Using a molecular phylogenetic tree we identified several differences shared among a subset of young repeats in one or the other species as candidates for species-specific LINE-1 variants. Species specificity was tested using oligonucleotide probes complementary to each putative species-specific variant. When hybridized to genomic DNAs, single-variant probes detected an expanded number of elements in the expected mouse. In the other species these probes detected a smaller number of matches consistent with the average rate of random divergence among LINE-1 elements. It was further found that the combination of two species-specific sequence differences in the same probe reduced the detection background in the wrong species below our detection limit.

Original languageEnglish (US)
Pages (from-to)635-643
Number of pages9
JournalJournal of Molecular Biology
Volume219
Issue number4
DOIs
StatePublished - Jun 20 1991

Keywords

  • DNA probes
  • LINE-1 repetitive DNA
  • Mus spretus
  • molecular phylogenetic trees
  • mouse genetics

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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