Synthesis of phosphotyrosyl-containing phosphopeptides by solid-phase peptide synthesis

Gustavo Zardeneta, Dali Chen, Susan T. Weintraub, Robert J. Klebe

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The synthesis of phosphotyrosine-containing phosphopeptides using solid-phase peptide synthesis (SPPS) techniques is described. We present the synthesis of a Boc-phosphotyrosine derivative, which when used with modifications of the conventional SPPS protocol permits the incorporation of phosphotyrosine into synthetic peptides. The resulting phosphopeptides were authenticated by fast atom bombardment mass spectrometry, amino acid analysis, and phosphate assay. Alkaline phosphatase was found to dephosphorylate synthetic phosphopeptides at different rates, supporting the potential use of these synthetic substrates for studies of phosphoprotein phosphatases. Synthesis of a phosphopeptide using the described protocol has several advantages over the preparation of phosphopeptides via enzymatic phosphorylation.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalAnalytical Biochemistry
Volume190
Issue number2
DOIs
StatePublished - Nov 1 1990

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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