Synthesis of mycocerosic acids from methylmalonyl coenzyme A by cell-free extracts of Mycobacterium tuberculosis var. bovis BCG

D. L. Rainwater, P. E. Kolattukudy

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    Abstract

    Multimethyl-branched acids extracted from the cells of Mycobacterium tuberculosis var. bovis BCG were identified by combined capillary gas-liquid chromatography and mass spectrometry. These mycocerosic acids consisted of the products expected from elongation of n-C18 and n-C20 primers with methylmalonyl-CoA. A soluble enzyme preparation from M. tuberculosis var. bovis BCG incorporated methylmalonyl-CoA into mycocerosic acids. This incorporation was partially dependent on the addition of arachidoyl-CoA and this primer affected product distribution, indicating elongation of the primer. Maximal incorporation of methylmalonyl-CoA required the presence of both NADPH and NADH together with ATP and Mg2+. Added CoA, FMN, acyl carrier protein, or bovine serum albumin had no effect on the activity in either the presence or absence of ATP and Mg2+. The pH optimum for mycocerosic acid synthesis was 6.2. Under these conditions, methylmalonyl-CoA was incorporated into very long acids which were identified as mycocerosic acids by radio gas-liquid chromatography.

    Original languageEnglish (US)
    Pages (from-to)2979-2985
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume258
    Issue number5
    Publication statusPublished - Jan 1 1983

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    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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