Synthesis of a novel hepatitis C virus protein by ribosomal frameshift

Zhenming Xu, Jinah Choi, T. S.Benedict Yen, Wen Lu, Anne Strohecker, Sugantha Govindarajan, David Chien, Mark J. Selby, Jing Hsiung Ou

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Hepatitis C virus (HCV) is an important human pathogen that affects ∼100 million people worldwide. Its RNA genome codes for a polyprotein, which is cleaved by viral and cellular proteases to produce at least 10 mature viral protein products. We report here the discovery of a novel HCV protein synthesized by ribosomal frameshift. This protein, which we named the F protein, is synthesized from the initiation codon of the polyprotein sequence followed by ribosomal frameshift into the -2/+1 reading frame. This ribosomal frameshift requires only codons 8-14 of the core protein-coding sequence, and the shift junction is located at or near codon 11. An F protein analog synthesized in vitro reacted with the sera of HCV patients but not with the sera of hepatitis B patients, indicating the expression of the F protein during natural HCV infection. This unexpected finding may open new avenues for the development of anti-HCV drugs.

Original languageEnglish (US)
Pages (from-to)3840-3848
Number of pages9
JournalEMBO Journal
Volume20
Issue number14
DOIs
StatePublished - Jul 16 2001
Externally publishedYes

Fingerprint

Ribosomal Frameshifting
Ribosomal Proteins
Viruses
Hepacivirus
Polyproteins
Proteins
Codon
Viral Proteins
Pathogens
Reading Frames
Initiator Codon
Virus Diseases
Hepatitis B
Serum
Peptide Hydrolases
Genes
RNA
Genome
Pharmaceutical Preparations

Keywords

  • HCV core protein
  • HCV F protein
  • Hepatitis C virus
  • Ribosomal frameshift

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Xu, Z., Choi, J., Yen, T. S. B., Lu, W., Strohecker, A., Govindarajan, S., ... Ou, J. H. (2001). Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. EMBO Journal, 20(14), 3840-3848. https://doi.org/10.1093/emboj/20.14.3840

Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. / Xu, Zhenming; Choi, Jinah; Yen, T. S.Benedict; Lu, Wen; Strohecker, Anne; Govindarajan, Sugantha; Chien, David; Selby, Mark J.; Ou, Jing Hsiung.

In: EMBO Journal, Vol. 20, No. 14, 16.07.2001, p. 3840-3848.

Research output: Contribution to journalArticle

Xu, Z, Choi, J, Yen, TSB, Lu, W, Strohecker, A, Govindarajan, S, Chien, D, Selby, MJ & Ou, JH 2001, 'Synthesis of a novel hepatitis C virus protein by ribosomal frameshift', EMBO Journal, vol. 20, no. 14, pp. 3840-3848. https://doi.org/10.1093/emboj/20.14.3840
Xu Z, Choi J, Yen TSB, Lu W, Strohecker A, Govindarajan S et al. Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. EMBO Journal. 2001 Jul 16;20(14):3840-3848. https://doi.org/10.1093/emboj/20.14.3840
Xu, Zhenming ; Choi, Jinah ; Yen, T. S.Benedict ; Lu, Wen ; Strohecker, Anne ; Govindarajan, Sugantha ; Chien, David ; Selby, Mark J. ; Ou, Jing Hsiung. / Synthesis of a novel hepatitis C virus protein by ribosomal frameshift. In: EMBO Journal. 2001 ; Vol. 20, No. 14. pp. 3840-3848.
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