A new haptenated derivative of α-galactosyl ceramide (α-GalCer) has been synthesized to assist in the study of the mechanism of T cell help for the production of B cell antibodies. Our synthetic route provides access to an amine intermediate which can easily be extended to generate an array of compounds, useful in various ongoing studies. Herein, we also describe the biological evaluation of the nitrophenyl (NP) haptenated α-GalCer and demonstrate its importance in such mechanistic studies. For instance, in vitro studies showed that NP-α-GalCer stimulates both T and B cell proliferation while in vivo studies in immunized mice showed the production of IgG anti-NP antibodies after exposure to NP-α-GalCer. The interpretation of these results helps toward a better understanding of T cell help for the production of antibodies.
|Original language||English (US)|
|Number of pages||7|
|State||Published - Apr 21 2010|
ASJC Scopus subject areas
- Biomedical Engineering
- Pharmaceutical Science
- Organic Chemistry