Synthesis and distribution of CARDS toxin during mycoplasma pneumoniae infection in a murine model

Thirumalai R Kannan, Jacqueline J. Coalson, Marianna Cagle, Oxana Musatovova, R. Doug Hardy, Joel B. Baseman

Research output: Contribution to journalArticle

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Abstract

Mice were infected with Mycoplasma pneumoniae and monitored for the synthesis and distribution of the unique adenosine diphosphate-ribosylating and vacuolating Community Acquired Respiratory Distress Syndrome (CARDS) toxin in bronchiolar lavage fluid (BALF) and lung. We noted direct relationships between the concentration of CARDS toxin and numbers of mycoplasma genomes in BALF and the degree of histologic pulmonary inflammation. Immunostaining of lungs revealed extensive colonization by mycoplasmas, including the detection of CARDS toxin in the corresponding inflamed airways. Lung lesion scores were higher during the early stages of infection, decreased gradually by day 14 postinfection, and reached substantially lower values at day 35. Infected mouse immunoglobulin (Ig) M and IgG titers were positive for CARDS toxin as well as for the major adhesin P1 of M. pneumoniae. These data reinforce the proposed pathogenic role of CARDS toxin in M. pneumoniae-mediated pathologies.

Original languageEnglish (US)
Pages (from-to)1596-1604
Number of pages9
JournalJournal of Infectious Diseases
Volume204
Issue number10
DOIs
StatePublished - Nov 15 2011

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Mycoplasma pneumoniae
Infection
Mycoplasma
Lung
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Adenosine Diphosphate
Immunoglobulin M
Pneumonia
Immunoglobulin G
Genome
Pathology

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Kannan, T. R., Coalson, J. J., Cagle, M., Musatovova, O., Hardy, R. D., & Baseman, J. B. (2011). Synthesis and distribution of CARDS toxin during mycoplasma pneumoniae infection in a murine model. Journal of Infectious Diseases, 204(10), 1596-1604. https://doi.org/10.1093/infdis/jir557

Synthesis and distribution of CARDS toxin during mycoplasma pneumoniae infection in a murine model. / Kannan, Thirumalai R; Coalson, Jacqueline J.; Cagle, Marianna; Musatovova, Oxana; Hardy, R. Doug; Baseman, Joel B.

In: Journal of Infectious Diseases, Vol. 204, No. 10, 15.11.2011, p. 1596-1604.

Research output: Contribution to journalArticle

Kannan, TR, Coalson, JJ, Cagle, M, Musatovova, O, Hardy, RD & Baseman, JB 2011, 'Synthesis and distribution of CARDS toxin during mycoplasma pneumoniae infection in a murine model', Journal of Infectious Diseases, vol. 204, no. 10, pp. 1596-1604. https://doi.org/10.1093/infdis/jir557
Kannan, Thirumalai R ; Coalson, Jacqueline J. ; Cagle, Marianna ; Musatovova, Oxana ; Hardy, R. Doug ; Baseman, Joel B. / Synthesis and distribution of CARDS toxin during mycoplasma pneumoniae infection in a murine model. In: Journal of Infectious Diseases. 2011 ; Vol. 204, No. 10. pp. 1596-1604.
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