Synergistically therapeutic effects of VEGF165 and angiopoietin-1 on ischemic rat myocardium

Xiang Liu, Yijiang Chen, Fumin Zhang, Lizhen Chen, Tuanzhu Ha, Xiang Gao, Chuanfu Li

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Purpose: The aim of this study was to determine whether the combination of 2 angiogenic growth factor, vascular endothelial growth factor 165(VEGF165) and angiopoietin-1 (Ang1), could increase angiogenesis and cardiomyocyte(CM) proliferation in an infarcted myocardium. Methods: Myocardial ischemia was induced in rats by ligation of the left anterior descending (LAD) coronary artery. Replication-deficient adenoviruses encoding VEGF165 (Ad-VEGF165), Ang1 (Ad-Ang1) or enhanced green fluorescence protein (Ad-EGFP) was injected into the ischemic myocardium immediately. Bromodexyuridine (BrdU) was administered intraperitoneally 1 week after ligation. One week later, the hearts were harvested and sectioned for hematoxylin-eosin (HE) and immunohistochemistry to evaluate densities of capillary, arteriole and double labelled BrdU(+) CM. M-mode echocardiography was used to evaluate the cardiac function. Results: Ang1 significantly increased collateral vessel formation. Both VEGF165 and Ang1 significantly increased densities of capillary and arteriole, as well as the number of double labelled BrdU(+) CM, and improved cardiac function. Conclusion: Our results suggest that the combination of VEGF165 and Ang1 can increase both myocardial angiogenesis and CM proliferation following myocardial ischemia in rats, leading to improved cardiac function.

Original languageEnglish (US)
Pages (from-to)95-101
Number of pages7
JournalScandinavian Cardiovascular Journal
Volume41
Issue number2
DOIs
StatePublished - Apr 24 2007
Externally publishedYes

Keywords

  • Angiopoietin-1
  • Cardiac regeneration
  • Gene therapy
  • Myocardial infarction
  • VEGF165

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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