TY - JOUR
T1 - Synergistic anti-oomycete effect of melatonin with a biofungicide against oomycetic black shank disease
AU - Zhang, Shumin
AU - Liu, Sen
AU - Zhang, Jiankui
AU - Reiter, Russel J.
AU - Wang, Ying
AU - Qiu, Dan
AU - Luo, Xiumei
AU - Khalid, A. Rehman
AU - Wang, Hanyan
AU - Feng, Li
AU - Lin, Zhenghong
AU - Ren, Maozhi
N1 - Funding Information:
This financial support from the Grants: National Key R & D Program for Crop Breeding (No. 2016YFD0100306); the National Natural Science Foundation of China (No. 31672206); Chongqing Social Undertakings and People's Livelihood Science and technology innovation Project: Tuber Crop Special New Varieties Creating and Clean & Safe Processing (No. CSTC2015shms-ztzx80003); Fundamental Research Funds for the Central Universities (No. 106112017CDJQJ29883) are acknowledged. Funding information National Key R & D Program for Crop Breeding, Grant/Award Number: No. 2016YFD0100306; the National Natural Science Foundation of China, Grant/Award Number: No. 31672206; Chongqing Social Undertakings and People's Livelihood Science and technology innovation Project: Tuber Crop Special New Varieties Creating and Clean & Safe Processing, Grant/Award Number: No. CSTC2015shms-ztzx80003; Fundamental Research Funds for the Central Universities, Grant/Award Number: No. 106112017CDJQJ29883
Funding Information:
National Key R & D Program for Crop Breeding, Grant/Award Number: No. 2016YFD0100306; the National Natural Science Foundation of China, Grant/Award Number: No. 31672206; Chongqing Social Undertakings and People’s Livelihood Science and technology innovation Project: Tuber Crop Special New Varieties Creating and Clean & Safe Processing, Grant/Award Number: No. CSTC2015shms-ztzx80003; Fundamental Research Funds for the Central Universities, Grant/Award Number: No. 106112017CDJQJ29883
Funding Information:
This financial support from the Grants: National Key R & D Program for Crop Breeding (No. 2016YFD0100306); the National Natural Science Foundation of China (No. 31672206); Chongqing Social Undertakings and People’s Livelihood Science and technology innovation Project: Tuber Crop Special New Varieties Creating and Clean & Safe Processing (No. CSTC2015shms-ztzx80003); Fundamental Research Funds for the Central Universities (No. 106112017CDJQJ29883) are acknowledged.
PY - 2018/9
Y1 - 2018/9
N2 - Human health, food safety, and agriculture have been threatened by oomycetic diseases caused by notorious pathogenic oomycetes. Chemical oomyceticides are the main approaches in control of pathogenic oomycetes. However, the overused chemical oomyceticides have resulted in serious environmental pollution and drug resistance. The eco-friendly bio-oomyceticides are required for sustainable development through screening synergistic drug combinations. In this study, Phytophthora nicotianae (P. nicotianae), as one of the most destructive oomycetic diseases in agriculture, was used as a model system to screen the novel bio-oomyceticides based on drug combination. The results showed that treatment of melatonin or ethylicin (IUPAC Name: 1-ethylsulfonylsulfanylethane) alone displayed similar phenotypes such as the inhibition of the hyphal growth, reduction of the cell viability, and suppression of the virulence of P. nicotianae. Importantly, melatonin and ethylicin shared the same targets of interfering with the amino acid metabolism, overexpressing apoptosis-inducing factor, and dysregulating the virulence-related genes. Furthermore, strong synergism against P. nicotianae was induced by combining melatonin with ethylicin. Under treatment of the combination of melatonin and ethylicin, the expression of genes associated with amino acid, the apoptosis-inducing factor, and the virulence-related genes was much more significantly dysregulated than that of single drug treatment. Thus, the tobacco black shank caused by P. nicotianae can be successfully controlled using the combination of melatonin and ethylicin. These observations suggest that the synergistic effect based on the combination of melatonin and ethylicin is an eco-friendly alternative for the control of the destructive oomycetic diseases.
AB - Human health, food safety, and agriculture have been threatened by oomycetic diseases caused by notorious pathogenic oomycetes. Chemical oomyceticides are the main approaches in control of pathogenic oomycetes. However, the overused chemical oomyceticides have resulted in serious environmental pollution and drug resistance. The eco-friendly bio-oomyceticides are required for sustainable development through screening synergistic drug combinations. In this study, Phytophthora nicotianae (P. nicotianae), as one of the most destructive oomycetic diseases in agriculture, was used as a model system to screen the novel bio-oomyceticides based on drug combination. The results showed that treatment of melatonin or ethylicin (IUPAC Name: 1-ethylsulfonylsulfanylethane) alone displayed similar phenotypes such as the inhibition of the hyphal growth, reduction of the cell viability, and suppression of the virulence of P. nicotianae. Importantly, melatonin and ethylicin shared the same targets of interfering with the amino acid metabolism, overexpressing apoptosis-inducing factor, and dysregulating the virulence-related genes. Furthermore, strong synergism against P. nicotianae was induced by combining melatonin with ethylicin. Under treatment of the combination of melatonin and ethylicin, the expression of genes associated with amino acid, the apoptosis-inducing factor, and the virulence-related genes was much more significantly dysregulated than that of single drug treatment. Thus, the tobacco black shank caused by P. nicotianae can be successfully controlled using the combination of melatonin and ethylicin. These observations suggest that the synergistic effect based on the combination of melatonin and ethylicin is an eco-friendly alternative for the control of the destructive oomycetic diseases.
KW - biofungicide
KW - drug combination
KW - melatonin
KW - pathogenic oomycetes
KW - synergistic inhibition
UR - http://www.scopus.com/inward/record.url?scp=85044868001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044868001&partnerID=8YFLogxK
U2 - 10.1111/jpi.12492
DO - 10.1111/jpi.12492
M3 - Article
C2 - 29575191
AN - SCOPUS:85044868001
VL - 65
JO - Journal of Pineal Research
JF - Journal of Pineal Research
SN - 0742-3098
IS - 2
M1 - e12492
ER -