Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes

Laurence S. Jouaville, François Ichas, Ekhson L. Holmuhamedov, Patricia Camacho, James D. Lechleiter

Research output: Contribution to journalLetterpeer-review

360 Scopus citations

Abstract

INXenopus oocytes, as well as other cells, inositol-l,4,5-tris-phosphate (Ins(l,4,5)P3)-induced Ca2+ release1-4 is an excitable process that generates propagating Ca2+ waves5-7 that annihilate upon collision8-12. The fundamental property responsible for excitability13 appears to be the Ca2+ dependency of the Ins(l,4,5)P3 receptor9. Here we report that Ins(l,4,5)P3-induced Ca2+ wave activity is strengthened by oxidizable substrates that energize mitochondria, increasing Ca2+ wave amplitude, velocity and interwave period. The effects of pyruvate/malate are blocked by ruthenium red at the Ca2+ uniporter, by rotenone at complex I, and by antimycin A at complex III, and are subsequently rescued at complex IV by ascorbate tetramethylphenylenediamine (TMPD)14. Our data reveal that potential-driven mitochondrial Ca2+ uptake is a major factor in the regulation of Ins(l,4,5)P3-induced Ca2+ release and clearly demonstrate a physiological role of mitochondria in intracellular Ca2+ signalling.

Original languageEnglish (US)
Pages (from-to)438-441
Number of pages4
JournalNature
Volume377
Issue number6548
DOIs
StatePublished - Oct 5 1995
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes'. Together they form a unique fingerprint.

Cite this