Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes

D. C. Thach, B. K. Agan, C. Olsen, J. Diao, B. Lin, J. Gomez, M. Jesse, M. Jenkins, R. Rowley, E. Hanson, C. Tibbetts, D. A. Stenger, Elizabeth A Walter, P. Demitry, T. Difato, R. Holliday, R. Kruzelock, L. Daum, D. Metzgar, D. Niemeyer & 23 others K. Russell, M. Archer, R. Bravo, N. Freed, J. Fuller, K. Gratwick, B. Johnson, E. Lawrence, C. Meador, H. Melgarejo, K. Mueller, D. Pearson, A. Purkayastha, J. Santiago, D. Seto, F. Stotler, J. Thornton, Z. Wang, D. Watson, S. Worthy, G. Vora, K. Grant, C. James

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Gene expression profiles permit analysis of host immune response at the transcriptome level. We used the Pax gene Blood RNA (PAX) System and Affymetrix microarrays (HG-U133A&B) to survey profiles in basic military trainees and to classify them as healthy, febrile respiratory illness (FRI) without adenovirus, FRI with adenovirus, and convalescent from FRI with adenovirus. We assessed quality metrics of RNA processing for microarrays. Class prediction analysis discovered nested sets of transcripts that could categorize the phenotypes with optimized accuracy of 99% (nonfebrile vs febrile, P<0.0005), 87% (healthy vs convalescent, P=0.001), and 91% (febrile without vs with adenovirus, P<0.0005). The discovered set for classification of nonfebrile vs febrile patients consisted of 40 transcripts with functions related to interferon induced genes, complement cascades, and TNF and IL1 signaling. The set of seven transcripts for distinguishing healthy vs convalescent individuals included those associated with ribosomal structure, humoral immunity, and cell adhesion. The set of 10 transcripts for distinguishing FRI without vs with adenovirus had functions related to interferon induced genes, IL1 receptor accessory protein, and cell interactions. These results are the first in vivo demonstration of classification of infectious diseases via host signature transcripts and move us towards using the transcriptome in biosurveillance.

Original languageEnglish (US)
Pages (from-to)588-595
Number of pages8
JournalGenes and Immunity
Volume6
Issue number7
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Transcriptome
Fever
Adenoviridae
Phenotype
Interferons
Biosurveillance
RNA
Genes
Interleukin-1 Receptors
Alternative Splicing
Humoral Immunity
Cell Adhesion
Cell Communication
Communicable Diseases
Proteins

Keywords

  • Infection phenotypes
  • Surveillance
  • Transcriptome

ASJC Scopus subject areas

  • Genetics(clinical)
  • Immunology
  • Genetics

Cite this

Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes. / Thach, D. C.; Agan, B. K.; Olsen, C.; Diao, J.; Lin, B.; Gomez, J.; Jesse, M.; Jenkins, M.; Rowley, R.; Hanson, E.; Tibbetts, C.; Stenger, D. A.; Walter, Elizabeth A; Demitry, P.; Difato, T.; Holliday, R.; Kruzelock, R.; Daum, L.; Metzgar, D.; Niemeyer, D.; Russell, K.; Archer, M.; Bravo, R.; Freed, N.; Fuller, J.; Gratwick, K.; Johnson, B.; Lawrence, E.; Meador, C.; Melgarejo, H.; Mueller, K.; Pearson, D.; Purkayastha, A.; Santiago, J.; Seto, D.; Stotler, F.; Thornton, J.; Wang, Z.; Watson, D.; Worthy, S.; Vora, G.; Grant, K.; James, C.

In: Genes and Immunity, Vol. 6, No. 7, 10.2005, p. 588-595.

Research output: Contribution to journalArticle

Thach, DC, Agan, BK, Olsen, C, Diao, J, Lin, B, Gomez, J, Jesse, M, Jenkins, M, Rowley, R, Hanson, E, Tibbetts, C, Stenger, DA, Walter, EA, Demitry, P, Difato, T, Holliday, R, Kruzelock, R, Daum, L, Metzgar, D, Niemeyer, D, Russell, K, Archer, M, Bravo, R, Freed, N, Fuller, J, Gratwick, K, Johnson, B, Lawrence, E, Meador, C, Melgarejo, H, Mueller, K, Pearson, D, Purkayastha, A, Santiago, J, Seto, D, Stotler, F, Thornton, J, Wang, Z, Watson, D, Worthy, S, Vora, G, Grant, K & James, C 2005, 'Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes', Genes and Immunity, vol. 6, no. 7, pp. 588-595. https://doi.org/10.1038/sj.gene.6364244
Thach, D. C. ; Agan, B. K. ; Olsen, C. ; Diao, J. ; Lin, B. ; Gomez, J. ; Jesse, M. ; Jenkins, M. ; Rowley, R. ; Hanson, E. ; Tibbetts, C. ; Stenger, D. A. ; Walter, Elizabeth A ; Demitry, P. ; Difato, T. ; Holliday, R. ; Kruzelock, R. ; Daum, L. ; Metzgar, D. ; Niemeyer, D. ; Russell, K. ; Archer, M. ; Bravo, R. ; Freed, N. ; Fuller, J. ; Gratwick, K. ; Johnson, B. ; Lawrence, E. ; Meador, C. ; Melgarejo, H. ; Mueller, K. ; Pearson, D. ; Purkayastha, A. ; Santiago, J. ; Seto, D. ; Stotler, F. ; Thornton, J. ; Wang, Z. ; Watson, D. ; Worthy, S. ; Vora, G. ; Grant, K. ; James, C. / Surveillance of transcriptomes in basic military trainees with normal, febrile respiratory illness, and convalescent phenotypes. In: Genes and Immunity. 2005 ; Vol. 6, No. 7. pp. 588-595.
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AU - Diao, J.

AU - Lin, B.

AU - Gomez, J.

AU - Jesse, M.

AU - Jenkins, M.

AU - Rowley, R.

AU - Hanson, E.

AU - Tibbetts, C.

AU - Stenger, D. A.

AU - Walter, Elizabeth A

AU - Demitry, P.

AU - Difato, T.

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AU - Fuller, J.

AU - Gratwick, K.

AU - Johnson, B.

AU - Lawrence, E.

AU - Meador, C.

AU - Melgarejo, H.

AU - Mueller, K.

AU - Pearson, D.

AU - Purkayastha, A.

AU - Santiago, J.

AU - Seto, D.

AU - Stotler, F.

AU - Thornton, J.

AU - Wang, Z.

AU - Watson, D.

AU - Worthy, S.

AU - Vora, G.

AU - Grant, K.

AU - James, C.

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