Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts

E. D. Rider, A. H. Jobe, T. Yamada, M. Ikegami, Steven R Seidner, L. Ruffini

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We studied the effect on surfactant metabolism of 8 h of mechanical ventilation at tidal volumes of 13 ± 0.3 ml/kg and very high tidal volumes of 28 ± 1.5 ml/kg, with and without added CO2, in the presence of an atrial right to left shunt in 4- to 8-day-old-lambs. Similarly aged, spontaneously breathing lambs were used as controls. Right to left atrial shunts were created by inflating a balloon in the right atrium after a Rashkind atrial septostomy, thus creating a stable, easily controlled atrial shunt. Radiolabeled surfactant phospholipid precursors were used to probe incorporation into and secretion of surfactant phosphatidylcholine, whereas intratracheally administered labeled natural surfactant was used to evaluate alveolar clearance. Protein leak from the vascular space to the lungs was measured using radioactive iodine-labeled albumins. At the end of the 8-h study period, tissue association of alveolar surfactant was significantly increased to 63% in the mechanically hyperventilated lambs as compared to 44% in those lambs mechanically ventilated but not hyperventilated (p < 0.05) and to 39% in the spontaneously breathing control animals (p < 0.05). No increased surfactant secretion or decreased compliance was detected with hyperventilation. However, the lambs had very large surfactant-saturated phosphatidylcholine pool sizes, and a large portion (50%) was already in the alveolar pool, even in the spontaneously breathing lambs. Precursor incorporation into saturated phosphatidylcholine was similar in all groups, and very low and comparable protein leaks were seen in the different groups of lambs. These experiments did not detect any adverse effects of 8 h of mechanical ventilation, large atrial shunts or mechanical hyperventilation on the surfactant system of newborn lambs.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalPediatric Research
Volume25
Issue number1
StatePublished - 1989
Externally publishedYes

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Hyperventilation
Surface-Active Agents
Phosphatidylcholines
Respiration
Tidal Volume
Artificial Respiration
Heart Atria
Iodine
Compliance
Blood Vessels
Albumins
Phospholipids
Proteins
Lung

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Rider, E. D., Jobe, A. H., Yamada, T., Ikegami, M., Seidner, S. R., & Ruffini, L. (1989). Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts. Pediatric Research, 25(1), 83-88.

Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts. / Rider, E. D.; Jobe, A. H.; Yamada, T.; Ikegami, M.; Seidner, Steven R; Ruffini, L.

In: Pediatric Research, Vol. 25, No. 1, 1989, p. 83-88.

Research output: Contribution to journalArticle

Rider, ED, Jobe, AH, Yamada, T, Ikegami, M, Seidner, SR & Ruffini, L 1989, 'Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts', Pediatric Research, vol. 25, no. 1, pp. 83-88.
Rider ED, Jobe AH, Yamada T, Ikegami M, Seidner SR, Ruffini L. Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts. Pediatric Research. 1989;25(1):83-88.
Rider, E. D. ; Jobe, A. H. ; Yamada, T. ; Ikegami, M. ; Seidner, Steven R ; Ruffini, L. / Surfactant metabolism during hyperventilation of newborn lambs with atrial right to left shunts. In: Pediatric Research. 1989 ; Vol. 25, No. 1. pp. 83-88.
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