Lymphocytes from patients with chronic lymphatic leukemia (CLL) carry B cell markers. To characterize the relationship between CLL cells and normal B lymphocytes, the authors examined binding of 5 purified plant lectins to normal human lymphocyte populations (mixed T and B cells, purified T, and purified B cells) and compared results with lectin binding to unseparated CLL lymphocytes. Normal T and B cells were separated on an immunoadsorbent column. Binding of 125I lectins showed equal numbers of surface receptors for a given lectin on each normal lymphocyte population: purified B, T, and mixed T + B cells. For these lymphocyte populations from normal subjects compared with lymphocytes from 5 untreated patients with CLL, binding sites x 106/cell (mean ± SE) were: E phytohemagglutinin (E PHA) 2.59 ±.72 vs 1.12 ± .32 for CLL: Wheat Germ Agglutinin (WGA) 34.45 ± .04 vs 20.5 ± 5.29; Concanavalin A (Con A) 1.24 ± .23 vs .33 ± .18; L PHA .63 ± .07 vs 1.47 ± .32 and Ricinis Communis Agglutinin (RCA I) 4.56 ±.50 vs 5.53 ±.76. Thus, compared to normal human lymphocyte populations, CLL cells had significantly fewer receptors for E PHA, WGA and Con A and significantly more receptors for L PHA. Binding of RCA was essentially unchanged. It is concluded that normal human T and B lymphocytes do not differ in receptor sites for 5 lectins; compared to normal B lymphocytes, CLL lymphocytes are characterized by extensive and consistent cell surface alterations detected by lectins and lectins are useful probes for characterization of cell surfaces in lymphoproliferative diseases.
|Original language||English (US)|
|State||Published - Jan 1 1975|
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