Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54

Stephen Van Komen; Galina Petukhova; Stefan Sigurdsson; Sabrina Stratton; Patrick Sung

doi:10.1016/S1097-2765(00)00055-1

Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54

Stephen Van Komen, Galina Petukhova, Stefan Sigurdsson, Sabrina Stratton, Patrick Sung

Research output: Contribution to journal › Article › peer-review

191 Scopus citations

Abstract

Yeast Rad51 recombinase has only minimal ability to form D loop. Addition of Rad54 renders D loop formation by Rad51 efficient, even when topologically relaxed DNA is used as substrate. Treatment of the nucleoprotein complex of Rad54 and relaxed DNA with topoisomerases reveals dynamic DNA remodeling to generate unconstrained negative and positive supercoils. DNA remodeling requires ATP hydrolysis by Rad54 and is stimulated by Rad51-DNA nucleoprotein complex. A marked sensitivity of DNA undergoing remodeling to P1 nuclease indicates that the negative supercoils produced lead to transient DNA strand separation. Thus, a specific interaction of Rad54 with the Rad51-ssDNA complex enhances the ability of the former to remodel DNA and allows the latter to harvest the negative supercoils generated for DNA joint formation.

Original language	English (US)
Pages (from-to)	563-572
Number of pages	10
Journal	Molecular Cell
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/S1097-2765(00)00055-1
State	Published - 2000

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(00)00055-1

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@article{a8c27ca739894e479fb58dfbca29b5c0,

title = "Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54",

abstract = "Yeast Rad51 recombinase has only minimal ability to form D loop. Addition of Rad54 renders D loop formation by Rad51 efficient, even when topologically relaxed DNA is used as substrate. Treatment of the nucleoprotein complex of Rad54 and relaxed DNA with topoisomerases reveals dynamic DNA remodeling to generate unconstrained negative and positive supercoils. DNA remodeling requires ATP hydrolysis by Rad54 and is stimulated by Rad51-DNA nucleoprotein complex. A marked sensitivity of DNA undergoing remodeling to P1 nuclease indicates that the negative supercoils produced lead to transient DNA strand separation. Thus, a specific interaction of Rad54 with the Rad51-ssDNA complex enhances the ability of the former to remodel DNA and allows the latter to harvest the negative supercoils generated for DNA joint formation.",

author = "{Van Komen}, Stephen and Galina Petukhova and Stefan Sigurdsson and Sabrina Stratton and Patrick Sung",

note = "Funding Information: We are grateful to John Diffley for discussions, to James Wang and Sue-Jane Chen and also to Yuk-Ching Tse-Dinh for their kind gifts of E. coli topoisomerase I, and to Michael Cox for the gift of RecA protein. This work was supported by National Institutes of Health research grants RO1GM57814 and RO1ES07061 and by training grants T32AG00165 from the National Institutes of Health (G. P.) and DAMD17-99-1-9402 from the US Army (S. S.).",

year = "2000",

doi = "10.1016/S1097-2765(00)00055-1",

language = "English (US)",

volume = "6",

pages = "563--572",

journal = "Molecular Cell",

issn = "1097-2765",

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T1 - Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54

AU - Van Komen, Stephen

AU - Petukhova, Galina

AU - Sigurdsson, Stefan

AU - Stratton, Sabrina

AU - Sung, Patrick

N1 - Funding Information: We are grateful to John Diffley for discussions, to James Wang and Sue-Jane Chen and also to Yuk-Ching Tse-Dinh for their kind gifts of E. coli topoisomerase I, and to Michael Cox for the gift of RecA protein. This work was supported by National Institutes of Health research grants RO1GM57814 and RO1ES07061 and by training grants T32AG00165 from the National Institutes of Health (G. P.) and DAMD17-99-1-9402 from the US Army (S. S.).

PY - 2000

Y1 - 2000

N2 - Yeast Rad51 recombinase has only minimal ability to form D loop. Addition of Rad54 renders D loop formation by Rad51 efficient, even when topologically relaxed DNA is used as substrate. Treatment of the nucleoprotein complex of Rad54 and relaxed DNA with topoisomerases reveals dynamic DNA remodeling to generate unconstrained negative and positive supercoils. DNA remodeling requires ATP hydrolysis by Rad54 and is stimulated by Rad51-DNA nucleoprotein complex. A marked sensitivity of DNA undergoing remodeling to P1 nuclease indicates that the negative supercoils produced lead to transient DNA strand separation. Thus, a specific interaction of Rad54 with the Rad51-ssDNA complex enhances the ability of the former to remodel DNA and allows the latter to harvest the negative supercoils generated for DNA joint formation.

AB - Yeast Rad51 recombinase has only minimal ability to form D loop. Addition of Rad54 renders D loop formation by Rad51 efficient, even when topologically relaxed DNA is used as substrate. Treatment of the nucleoprotein complex of Rad54 and relaxed DNA with topoisomerases reveals dynamic DNA remodeling to generate unconstrained negative and positive supercoils. DNA remodeling requires ATP hydrolysis by Rad54 and is stimulated by Rad51-DNA nucleoprotein complex. A marked sensitivity of DNA undergoing remodeling to P1 nuclease indicates that the negative supercoils produced lead to transient DNA strand separation. Thus, a specific interaction of Rad54 with the Rad51-ssDNA complex enhances the ability of the former to remodel DNA and allows the latter to harvest the negative supercoils generated for DNA joint formation.

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JO - Molecular Cell

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Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54

Abstract

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