[3H]Nisoxetine: a new radioligand for norepinephrine uptake sites in brain

Shanaz M. Tejani-Butt; David J. Brunswick; Alan Frazer

doi:10.1016/0014-2999(90)94155-Q

[³H]Nisoxetine: a new radioligand for norepinephrine uptake sites in brain

Shanaz M. Tejani-Butt, David J. Brunswick, Alan Frazer

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

[³H]Nisoxetine binds with high affinity (K_d = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [³H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90% of total binding at its K_d concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95% decrease in binding. [³H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.

Original language	English (US)
Pages (from-to)	239-243
Number of pages	5
Journal	European Journal of Pharmacology
Volume	191
Issue number	2
DOIs	https://doi.org/10.1016/0014-2999(90)94155-Q
State	Published - Nov 27 1990
Externally published	Yes

Keywords

Norepinephrine
Uptake sites
[H]Nisoxetine

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1016/0014-2999(90)94155-Q

Cite this

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title = "[3H]Nisoxetine: a new radioligand for norepinephrine uptake sites in brain",

abstract = "[3H]Nisoxetine binds with high affinity (Kd = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [3H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90\% of total binding at its Kd concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95\% decrease in binding. [3H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.",

keywords = "Norepinephrine, Uptake sites, [H]Nisoxetine",

author = "Tejani-Butt, \{Shanaz M.\} and Brunswick, \{David J.\} and Alan Frazer",

note = "Funding Information: This work was supportebdy researcfhun ds from the Department of Veterans Affairs and USPHS Grant DA 05137.",

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T1 - [3H]Nisoxetine

T2 - a new radioligand for norepinephrine uptake sites in brain

AU - Tejani-Butt, Shanaz M.

AU - Brunswick, David J.

AU - Frazer, Alan

N1 - Funding Information: This work was supportebdy researcfhun ds from the Department of Veterans Affairs and USPHS Grant DA 05137.

PY - 1990/11/27

Y1 - 1990/11/27

N2 - [3H]Nisoxetine binds with high affinity (Kd = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [3H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90% of total binding at its Kd concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95% decrease in binding. [3H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.

AB - [3H]Nisoxetine binds with high affinity (Kd = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [3H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90% of total binding at its Kd concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95% decrease in binding. [3H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.

KW - Norepinephrine

KW - Uptake sites

KW - [H]Nisoxetine

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