[3H]Nisoxetine: a new radioligand for norepinephrine uptake sites in brain

Shanaz M. Tejani-Butt, David J. Brunswick, Alan Frazer

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

[3H]Nisoxetine binds with high affinity (Kd = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [3H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90% of total binding at its Kd concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95% decrease in binding. [3H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.

Original languageEnglish (US)
Pages (from-to)239-243
Number of pages5
JournalEuropean Journal of Pharmacology
Volume191
Issue number2
DOIs
StatePublished - Nov 27 1990
Externally publishedYes

Keywords

  • Norepinephrine
  • Uptake sites
  • [H]Nisoxetine

ASJC Scopus subject areas

  • Pharmacology

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