Abstract
[3H]Nisoxetine binds with high affinity (Kd = 0.7 nM) and selectivity to a homogenous population of sites associated with the uptake of norepinephrine. Specific [3H]nisoxetine binding to rat cortical homogenates was saturable, sodium-dependent and averaged 90% of total binding at its Kd concentration. Pretreatment with the neurotoxin DSP-4 resulted in 95% decrease in binding. [3H]Nisoxetine exhibits superior properties to radioligands previously used and appears to be the radioligand of choice for studies involving uptake sites for norepinephrine.
Original language | English (US) |
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Pages (from-to) | 239-243 |
Number of pages | 5 |
Journal | European Journal of Pharmacology |
Volume | 191 |
Issue number | 2 |
DOIs | |
State | Published - Nov 27 1990 |
Externally published | Yes |
Keywords
- Norepinephrine
- Uptake sites
- [H]Nisoxetine
ASJC Scopus subject areas
- Pharmacology