31P nuclear magnetic resonance spectroscopy studies of substrate and product binding to fructose-1,6-bisphosphatase

Feng Liu, Herbert J. Fromm

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The enzymatic hydrolysis of fructose 1,6-bisphosphate (Fru-1,6-P2) to fructose 6-phosphate (Fru-6-P) and inorganic phosphate (Pi), which is catalyzed by fructose-1,6-bisphosphatase, has been studied by 31p nuclear magnetic resonance spectroscopy (NMR). At pH 7.5 and 15 °C, the equilibrium constant for the central complex K′eq = [E · Fru-6-P·P-i]/[E·Fru-1,6-P2 · H2O] is about 2. This observation is in harmony with results obtained with a number of Bi Bi enzyme systems for the determination of K′eq in which a variety of experimental techniques were used (Knowles, J. R. (1980) Annu. Rev. Biochem. 49, 877-919). Significant changes in 31P NMR chemical shifts were observed for both the substrate, Fru-1,6-P2, and the product, Fru-6-P, when bound to the enzyme relative to ligand free in solution. The chemical shifts of the substrate and product were altered further in the presence of Mg2+, the catalytic divalent metal ion. The chemical shifts caused by the addition of metal ion can be reversed in the presence of trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA) or AMP. In the presence of the metal ion chelator or the nucleotide, the substrate had a chemical shift that was about the same as that observed in the absence of metal ion. On the basis of these observations we suggest that AMP and CDTA exhibit similar effects, i.e. they both remove the catalytic metal ion from the enzyme. This finding is supportive of the suggestion (Scheffler, J. E., and Fromm, H. J. (1986) Biochemistry 25, 6659-6665; Liu, F., and Fromm, H. J. (1990) J. Biol. Chem. 265, 7401-7406) that the role of AMP in the regulation of fructose-1,6-bisphosphatase is to prevent binding of the divalent metal activator to the enzyme.

Original languageEnglish (US)
Pages (from-to)11774-11778
Number of pages5
JournalJournal of Biological Chemistry
Volume266
Issue number18
StatePublished - 1991
Externally publishedYes

Fingerprint

Fructose-Bisphosphatase
Nuclear magnetic resonance spectroscopy
Metal ions
Chemical shift
Magnetic Resonance Spectroscopy
Metals
Adenosine Monophosphate
Ions
Substrates
Enzymes
Biochemistry
Enzyme Activators
Enzymatic hydrolysis
Equilibrium constants
Chelating Agents
Nucleotides
Phosphates
Ligands
Hydrolysis
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

31P nuclear magnetic resonance spectroscopy studies of substrate and product binding to fructose-1,6-bisphosphatase. / Liu, Feng; Fromm, Herbert J.

In: Journal of Biological Chemistry, Vol. 266, No. 18, 1991, p. 11774-11778.

Research output: Contribution to journalArticle

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abstract = "The enzymatic hydrolysis of fructose 1,6-bisphosphate (Fru-1,6-P2) to fructose 6-phosphate (Fru-6-P) and inorganic phosphate (Pi), which is catalyzed by fructose-1,6-bisphosphatase, has been studied by 31p nuclear magnetic resonance spectroscopy (NMR). At pH 7.5 and 15 °C, the equilibrium constant for the central complex K′eq = [E · Fru-6-P·P-i]/[E·Fru-1,6-P2 · H2O] is about 2. This observation is in harmony with results obtained with a number of Bi Bi enzyme systems for the determination of K′eq in which a variety of experimental techniques were used (Knowles, J. R. (1980) Annu. Rev. Biochem. 49, 877-919). Significant changes in 31P NMR chemical shifts were observed for both the substrate, Fru-1,6-P2, and the product, Fru-6-P, when bound to the enzyme relative to ligand free in solution. The chemical shifts of the substrate and product were altered further in the presence of Mg2+, the catalytic divalent metal ion. The chemical shifts caused by the addition of metal ion can be reversed in the presence of trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA) or AMP. In the presence of the metal ion chelator or the nucleotide, the substrate had a chemical shift that was about the same as that observed in the absence of metal ion. On the basis of these observations we suggest that AMP and CDTA exhibit similar effects, i.e. they both remove the catalytic metal ion from the enzyme. This finding is supportive of the suggestion (Scheffler, J. E., and Fromm, H. J. (1986) Biochemistry 25, 6659-6665; Liu, F., and Fromm, H. J. (1990) J. Biol. Chem. 265, 7401-7406) that the role of AMP in the regulation of fructose-1,6-bisphosphatase is to prevent binding of the divalent metal activator to the enzyme.",
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N2 - The enzymatic hydrolysis of fructose 1,6-bisphosphate (Fru-1,6-P2) to fructose 6-phosphate (Fru-6-P) and inorganic phosphate (Pi), which is catalyzed by fructose-1,6-bisphosphatase, has been studied by 31p nuclear magnetic resonance spectroscopy (NMR). At pH 7.5 and 15 °C, the equilibrium constant for the central complex K′eq = [E · Fru-6-P·P-i]/[E·Fru-1,6-P2 · H2O] is about 2. This observation is in harmony with results obtained with a number of Bi Bi enzyme systems for the determination of K′eq in which a variety of experimental techniques were used (Knowles, J. R. (1980) Annu. Rev. Biochem. 49, 877-919). Significant changes in 31P NMR chemical shifts were observed for both the substrate, Fru-1,6-P2, and the product, Fru-6-P, when bound to the enzyme relative to ligand free in solution. The chemical shifts of the substrate and product were altered further in the presence of Mg2+, the catalytic divalent metal ion. The chemical shifts caused by the addition of metal ion can be reversed in the presence of trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA) or AMP. In the presence of the metal ion chelator or the nucleotide, the substrate had a chemical shift that was about the same as that observed in the absence of metal ion. On the basis of these observations we suggest that AMP and CDTA exhibit similar effects, i.e. they both remove the catalytic metal ion from the enzyme. This finding is supportive of the suggestion (Scheffler, J. E., and Fromm, H. J. (1986) Biochemistry 25, 6659-6665; Liu, F., and Fromm, H. J. (1990) J. Biol. Chem. 265, 7401-7406) that the role of AMP in the regulation of fructose-1,6-bisphosphatase is to prevent binding of the divalent metal activator to the enzyme.

AB - The enzymatic hydrolysis of fructose 1,6-bisphosphate (Fru-1,6-P2) to fructose 6-phosphate (Fru-6-P) and inorganic phosphate (Pi), which is catalyzed by fructose-1,6-bisphosphatase, has been studied by 31p nuclear magnetic resonance spectroscopy (NMR). At pH 7.5 and 15 °C, the equilibrium constant for the central complex K′eq = [E · Fru-6-P·P-i]/[E·Fru-1,6-P2 · H2O] is about 2. This observation is in harmony with results obtained with a number of Bi Bi enzyme systems for the determination of K′eq in which a variety of experimental techniques were used (Knowles, J. R. (1980) Annu. Rev. Biochem. 49, 877-919). Significant changes in 31P NMR chemical shifts were observed for both the substrate, Fru-1,6-P2, and the product, Fru-6-P, when bound to the enzyme relative to ligand free in solution. The chemical shifts of the substrate and product were altered further in the presence of Mg2+, the catalytic divalent metal ion. The chemical shifts caused by the addition of metal ion can be reversed in the presence of trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA) or AMP. In the presence of the metal ion chelator or the nucleotide, the substrate had a chemical shift that was about the same as that observed in the absence of metal ion. On the basis of these observations we suggest that AMP and CDTA exhibit similar effects, i.e. they both remove the catalytic metal ion from the enzyme. This finding is supportive of the suggestion (Scheffler, J. E., and Fromm, H. J. (1986) Biochemistry 25, 6659-6665; Liu, F., and Fromm, H. J. (1990) J. Biol. Chem. 265, 7401-7406) that the role of AMP in the regulation of fructose-1,6-bisphosphatase is to prevent binding of the divalent metal activator to the enzyme.

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