31P NMR measurement of ATP synthesis rate in perfused intact rat hearts

P. Kingsley-Hickman; E. Y. Sako; P. A. Andreone; J. A. St. Cyr; S. Michurski; J. E. Foker; A. H.L. From; M. Petein; K. Ugurbil

doi:10.1016/0014-5793(86)81204-2

³¹P NMR measurement of ATP synthesis rate in perfused intact rat hearts

P. Kingsley-Hickman
, E. Y. Sako
, P. A. Andreone
, J. A. St. Cyr
, S. Michurski
, J. E. Foker
, A. H.L. From
, M. Petein
, K. Ugurbil

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Using ³¹P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 10³ mmHg · min^-1 and consuming O₂; at a rate of 35 ± 2 μmol O₂ · min^-1. (g dry wt)^-1, at 37°C. This rate was 7.2 ± 0.9 μmol · s^-1 · (g dry wt)^-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

Original language	English (US)
Pages (from-to)	159-163
Number of pages	5
Journal	FEBS Letters
Volume	198
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)81204-2
State	Published - Mar 17 1986
Externally published	Yes

Keywords

( Rat myocardium)
ATPase
P-NMR
Saturation transfer

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

Access to Document

10.1016/0014-5793(86)81204-2

Cite this

@article{8b51e7a0b0fe4721938dcd1a6a362bdb,

title = "31P NMR measurement of ATP synthesis rate in perfused intact rat hearts",

abstract = "Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 103 mmHg · min-1 and consuming O2; at a rate of 35 ± 2 μmol O2 · min-1. (g dry wt)-1, at 37°C. This rate was 7.2 ± 0.9 μmol · s-1 · (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.",

keywords = "( Rat myocardium), ATPase, P-NMR, Saturation transfer",

author = "P. Kingsley-Hickman and Sako, \{E. Y.\} and Andreone, \{P. A.\} and \{St. Cyr\}, \{J. A.\} and S. Michurski and Foker, \{J. E.\} and From, \{A. H.L.\} and M. Petein and K. Ugurbil",

note = "Funding Information: This researchw as supported by NIH grants fK04 HL01241, ROl HL33600 and ROI HL26640, VeteransA dministrationM edical ResearchF unds, and Karl-Thomae GmbH (Biberach an der Riff, FRG).",

year = "1986",

month = mar,

day = "17",

doi = "10.1016/0014-5793(86)81204-2",

language = "English (US)",

volume = "198",

pages = "159--163",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

TY - JOUR

T1 - 31P NMR measurement of ATP synthesis rate in perfused intact rat hearts

AU - Kingsley-Hickman, P.

AU - Sako, E. Y.

AU - Andreone, P. A.

AU - St. Cyr, J. A.

AU - Michurski, S.

AU - Foker, J. E.

AU - From, A. H.L.

AU - Petein, M.

AU - Ugurbil, K.

N1 - Funding Information: This researchw as supported by NIH grants fK04 HL01241, ROl HL33600 and ROI HL26640, VeteransA dministrationM edical ResearchF unds, and Karl-Thomae GmbH (Biberach an der Riff, FRG).

PY - 1986/3/17

Y1 - 1986/3/17

N2 - Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 103 mmHg · min-1 and consuming O2; at a rate of 35 ± 2 μmol O2 · min-1. (g dry wt)-1, at 37°C. This rate was 7.2 ± 0.9 μmol · s-1 · (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

AB - Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 ± 2.5 (SE) × 103 mmHg · min-1 and consuming O2; at a rate of 35 ± 2 μmol O2 · min-1. (g dry wt)-1, at 37°C. This rate was 7.2 ± 0.9 μmol · s-1 · (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 ± 0.9. These date show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.

KW - ( Rat myocardium)

KW - ATPase

KW - P-NMR

KW - Saturation transfer

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DO - 10.1016/0014-5793(86)81204-2

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AN - SCOPUS:0022558365

SN - 0014-5793

VL - 198

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EP - 163

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