[186Re]Liposomal doxorubicin (Doxil): in vitro stability, pharmacokinetics, imaging and biodistribution in a head and neck squamous cell carcinoma xenograft model

Anuradha Soundararajan, Ande Bao, William T. Phillips, Ricardo Perez, Beth A. Goins

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

The purpose of this study was to determine the feasibility of radiolabeling liposomal doxorubicin (Doxil) for cancer chemoradionuclide therapy by directly loading the therapeutic radionuclide rhenium-186 (186Re) into the liposome interior. The pharmacokinetics, imaging and biodistribution of [186Re]Doxil (555 MBq/kg) and control [186Re]polyethylene glycol (PEG) liposomes (555 MBq/kg) were determined after intravenous administration in a head and neck cancer xenograft model in nude rats. [186Re]Doxil and [186Re]PEG liposomes were radiolabeled using [186Re]N,N-bis(2-mercaptoethyl)-N′,N′-diethylethylenediamine. 186Re labeling efficiency was 76.1±8.3% with Doxil. The in vitro serum stability of [186Re]Doxil at 37°C was 38.06±12.13% at 24 h. Pharmacokinetic studies revealed that [186Re]Doxil had a two-phase blood clearance with half clearance times of 0.8 and 28.2 h. Images acquired over 120 h showed that [186Re]Doxil had slow blood clearance, low liver accumulation and increasing spleen accumulation. The biodistribution study at 120 h indicated that the percentage of injected dose (%ID) in the blood and tumor for [186Re]Doxil was 20-fold higher than that of [186Re]PEG liposomes. The %ID values in the kidney and liver were not significantly different between [186Re]Doxil and [186Re]PEG liposomes. These results suggest that the long circulation and prolonged bioavailability of [186Re]Doxil could potentially deliver high concentrations of both doxorubicin and 186Re to tumor when encapsulated in the same liposome vehicle.

Original languageEnglish (US)
Pages (from-to)515-524
Number of pages10
JournalNuclear Medicine and Biology
Volume36
Issue number5
DOIs
StatePublished - Jul 1 2009

Keywords

  • Biodistribution
  • Doxil
  • Liposome
  • Nanoparticle
  • Pharmacokinetics
  • Rhenium-186
  • SPECT/CT

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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