Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association

Prabha Sarangi, Zdenka Bartosova, Veronika Altmannova, Cory Holland, Melita Chavdarova, Sang Lee, Lumir Krejci, Xiaolan Zhao

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae Rad1-Rad10 complex is a conserved, structure-specific endonuclease important for repairing multiple types of DNA lesions. Upon recruitment to lesion sites, Rad1-Rad10 removes damaged sequences, enabling subsequent gap filling and ligation. Acting at mid-steps of repair, the association and dissociation of Rad1-Rad10 with DNA can influence repair efficiency. We show that genotoxin-enhanced Rad1 sumoylation occurs after the nuclease is recruited to lesion sites. A single lysine outside Rad1's nuclease and Rad10-binding domains is sumoylated in vivo and in vitro. Mutation of this site to arginine abolishes Rad1 sumoylation and impairs Rad1-mediated repair at high doses of DNA damage, but sustains the repair of a single double-stranded break. The timing of Rad1 sumoylation and the phenotype bias toward high lesion loads point to a post-incision role for sumoylation, possibly affecting Rad1 dissociation from DNA. Indeed, biochemical examination shows that sumoylation of Rad1 decreases the complex's affinity for DNA without affecting other protein properties. These findings suggest a model whereby sumoylation of Rad1 promotes its disengagement from DNA after nuclease cleavage, allowing it to efficiently attend to large numbers of DNA lesions.

Original languageEnglish (US)
Pages (from-to)6393-6404
Number of pages12
JournalNucleic Acids Research
Volume42
Issue number10
DOIs
StatePublished - 2014

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Sumoylation
DNA Repair
DNA
Deoxyribonucleases
Endonucleases
Mutagens
Lysine
DNA Damage
Ligation
Saccharomyces cerevisiae
Arginine
Phenotype
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

Sarangi, P., Bartosova, Z., Altmannova, V., Holland, C., Chavdarova, M., Lee, S., ... Zhao, X. (2014). Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association. Nucleic Acids Research, 42(10), 6393-6404. https://doi.org/10.1093/nar/gku300

Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association. / Sarangi, Prabha; Bartosova, Zdenka; Altmannova, Veronika; Holland, Cory; Chavdarova, Melita; Lee, Sang; Krejci, Lumir; Zhao, Xiaolan.

In: Nucleic Acids Research, Vol. 42, No. 10, 2014, p. 6393-6404.

Research output: Contribution to journalArticle

Sarangi, P, Bartosova, Z, Altmannova, V, Holland, C, Chavdarova, M, Lee, S, Krejci, L & Zhao, X 2014, 'Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association', Nucleic Acids Research, vol. 42, no. 10, pp. 6393-6404. https://doi.org/10.1093/nar/gku300
Sarangi, Prabha ; Bartosova, Zdenka ; Altmannova, Veronika ; Holland, Cory ; Chavdarova, Melita ; Lee, Sang ; Krejci, Lumir ; Zhao, Xiaolan. / Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association. In: Nucleic Acids Research. 2014 ; Vol. 42, No. 10. pp. 6393-6404.
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