Succinate semialdehyde dehydrogenase deficiency does not down-regulate γ-hydroxybutyric acid binding sites in the mouse brain

Ashok K. Mehta, Georgianna G. Gould, Maneesh Gupta, Lawrence P. Carter, K. Michael Gibson, Maharaj K. Ticku

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We investigated whether succinate semialdehyde dehydrogenase deficiency alters γ-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [3H]NCS-382 revealed no significant changes in the affinity (Kd), receptor density (Bmax), or displacement potency (IC50) in various brain regions of Aldh5a1-/- vs. Aldh5a1+/+ mice.

Original languageEnglish (US)
Pages (from-to)86-89
Number of pages4
JournalMolecular Genetics and Metabolism
Volume88
Issue number1
DOIs
StatePublished - May 1 2006

Keywords

  • Autoradiography
  • GHB receptor
  • Membrane homogenate binding
  • SSADH deficiency
  • [H]NCS-382

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Endocrinology

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