Succinate semialdehyde dehydrogenase deficiency does not down-regulate γ-hydroxybutyric acid binding sites in the mouse brain

Ashok K. Mehta; Georgianna G. Gould; Maneesh Gupta; Lawrence P. Carter; K. Michael Gibson; Maharaj K. Ticku

doi:10.1016/j.ymgme.2005.11.012

Succinate semialdehyde dehydrogenase deficiency does not down-regulate γ-hydroxybutyric acid binding sites in the mouse brain

Ashok K. Mehta, Georgianna G. Gould, Maneesh Gupta, Lawrence P. Carter, K. Michael Gibson, Maharaj K. Ticku

Cellular & Integrative Physiology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We investigated whether succinate semialdehyde dehydrogenase deficiency alters γ-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [³H]NCS-382 revealed no significant changes in the affinity (K_d), receptor density (B_max), or displacement potency (IC₅₀) in various brain regions of Aldh5a1^-/- vs. Aldh5a1^+/+ mice.

Original language	English (US)
Pages (from-to)	86-89
Number of pages	4
Journal	Molecular Genetics and Metabolism
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.ymgme.2005.11.012
State	Published - May 1 2006

Keywords

Autoradiography
GHB receptor
Membrane homogenate binding
SSADH deficiency
[H]NCS-382

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Molecular Biology
Genetics
Endocrinology

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Succinate semialdehyde dehydrogenase deficiency does not down-regulate γ-hydroxybutyric acid binding sites in the mouse brain. / Mehta, Ashok K.; Gould, Georgianna G.; Gupta, Maneesh; Carter, Lawrence P.; Gibson, K. Michael; Ticku, Maharaj K.

In: Molecular Genetics and Metabolism, Vol. 88, No. 1, 01.05.2006, p. 86-89.

Research output: Contribution to journal › Article › peer-review

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