Abstract
We investigated whether succinate semialdehyde dehydrogenase deficiency alters γ-hydroxybutyric acid (GHB) receptor characteristics due to elevation of GHB levels in the mouse brain. The membrane homogenate binding and quantitative autoradiography using [3H]NCS-382 revealed no significant changes in the affinity (Kd), receptor density (Bmax), or displacement potency (IC50) in various brain regions of Aldh5a1-/- vs. Aldh5a1+/+ mice.
Original language | English (US) |
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Pages (from-to) | 86-89 |
Number of pages | 4 |
Journal | Molecular Genetics and Metabolism |
Volume | 88 |
Issue number | 1 |
DOIs | |
State | Published - May 1 2006 |
Keywords
- Autoradiography
- GHB receptor
- Membrane homogenate binding
- SSADH deficiency
- [H]NCS-382
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Molecular Biology
- Genetics
- Endocrinology