Successful transient introduction of Leishmania RNA virus into a virally infected and an uninfected strain of Leishmania

T. C. Armstrong, M. C. Keenan, G. Widmer, J. L. Patterson

    Research output: Contribution to journalArticlepeer-review

    16 Scopus citations

    Abstract

    Viruses of Leishmania have recently been identified and characterized. These viruses are consistently double-stranded RNA viruses of ≃5 kb. To date, they have not been reported to exist outside their protozoan host, nor have they been shown to be infectious. We report here the ability to transiently transfer these viruses to two strains of Leishmania, one previously infected and one that did not previously carry a virus. A PCR- based assay was used to detect viral negative-stranded RNA. Input RNA was ruled out as the source of template because a replication-incompetent (UV inactivated) virus was not detectable after transfer into Leishmania.

    Original languageEnglish (US)
    Pages (from-to)1736-1740
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume90
    Issue number5
    DOIs
    StatePublished - 1993

    ASJC Scopus subject areas

    • General

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