Substrate effects in the post-ischemic myocardium

Edward Y Sako, Peter B. Kingsley-Hickman, Arthur H L From, Kamil Ugurbil, John E. Foker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A study was undertaken to examine the effects of glucose versus pyruvate as the sole substrate following severe myocardial ischemia. Glycolysis usually contributes only a small amount to total ATP production and may be rate limiting in providing tricarboxcylic acid (TCA) cycle substrates. Consequently, pyruvate may be a more effective substrate by bypassing glycolysis to feed directly to the TCA cycle and oxidative phosphorylation. Isolated rat hearts were studied in a retrograde (Langendorff) perfusion apparatus while in an NMR spectrometer. Rate pressure product (RPP), myocardial oxygen consumption (MVO2), and the unidirectional Pi → ATP rate were measured in control and postischemic hearts with or without the inotrope dobutamine. The unidirectional Pi → ATP rate was higher in the glucose than the pyruvate hearts and the difference increased further postischemia. This increase over that of the pyruvate hearts has been attributed to a glycolytic component of ATP metabolism. Oxygen consumption was higher in pyruvate hearts at equivalent levels of performance. It thus appears that the glycolysis rate is significant and may be elevated following severe myocardial ischemia. Perfusion with pyruvate requires increased rates of oxidative phosphorylation to make up for the loss of glycolytically produced ATP. Optimal postischemic substrate delivery may require several compounds, one of which should be glucose.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalJournal of Surgical Research
Volume44
Issue number4
DOIs
StatePublished - 1988
Externally publishedYes

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Pyruvic Acid
Myocardium
Adenosine Triphosphate
Glycolysis
Oxidative Phosphorylation
Glucose
Oxygen Consumption
Myocardial Ischemia
Perfusion
Acids
Dobutamine
Pressure

ASJC Scopus subject areas

  • Surgery

Cite this

Sako, E. Y., Kingsley-Hickman, P. B., From, A. H. L., Ugurbil, K., & Foker, J. E. (1988). Substrate effects in the post-ischemic myocardium. Journal of Surgical Research, 44(4), 430-435. https://doi.org/10.1016/0022-4804(88)90186-2

Substrate effects in the post-ischemic myocardium. / Sako, Edward Y; Kingsley-Hickman, Peter B.; From, Arthur H L; Ugurbil, Kamil; Foker, John E.

In: Journal of Surgical Research, Vol. 44, No. 4, 1988, p. 430-435.

Research output: Contribution to journalArticle

Sako, EY, Kingsley-Hickman, PB, From, AHL, Ugurbil, K & Foker, JE 1988, 'Substrate effects in the post-ischemic myocardium', Journal of Surgical Research, vol. 44, no. 4, pp. 430-435. https://doi.org/10.1016/0022-4804(88)90186-2
Sako, Edward Y ; Kingsley-Hickman, Peter B. ; From, Arthur H L ; Ugurbil, Kamil ; Foker, John E. / Substrate effects in the post-ischemic myocardium. In: Journal of Surgical Research. 1988 ; Vol. 44, No. 4. pp. 430-435.
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