TY - JOUR
T1 - Substance P and somatostatin inhibit calcium channels in rat sympathetic neurons via different G protein pathwavs
AU - Shapiro, Mark S.
AU - Hille, Bertil
N1 - Funding Information:
We thank Drs. J. Herrington, K. Mackie, Y. B. Park, A. Tse, F. Viana, and L. P. Wollmuth for reading the manuscript, D. Anderson for building apparatus, L. Miller for technical help, and Pfizer, Inc. for their gift of CP-96,345. This work was sup ported by National Institutes of Health grant NS 08174, a McKnight Neuroscience Research Award, a grant from the W. M. Keck Foundation, and a National Research Service Award, NS 07332, to M. S. S. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisemenf” in accordance with 18 USC Section 1734 solely to indicate fact.
PY - 1993/1
Y1 - 1993/1
N2 - We studied inhibition of N-type Ca2+ channels in rat superior cervical ganglion neurons by substance P (SP) and somatostatin-14 (Som). In whole-cell clamp, 70 of 82 acutely dissociated neurons showed inhibition (mean 37%) by 500 nM SP, and 54 of 61 showed inhibition by 240 nM Som (mean 57% ). Pertussis toxin (PTX) blocked Som but not SP inhibition; intracellular dialysis with 2 mM GDP-β-S attenuated inhibition with either peptide. Inhibition was voltage dependent with Som but not with SP. Neurokinin A (1 μM) or B was without effect, implicating NK1 tachykinin receptors. In cell-attached patches with bath-applied drugs, to test for a diffusible messenger, inhibition by SP or Som was only 8%. Thus, SP signaling is voltage independent and PTX insensitive; Som inhibition is voltage dependent and PTX sensitive; and both are membrane delimited.
AB - We studied inhibition of N-type Ca2+ channels in rat superior cervical ganglion neurons by substance P (SP) and somatostatin-14 (Som). In whole-cell clamp, 70 of 82 acutely dissociated neurons showed inhibition (mean 37%) by 500 nM SP, and 54 of 61 showed inhibition by 240 nM Som (mean 57% ). Pertussis toxin (PTX) blocked Som but not SP inhibition; intracellular dialysis with 2 mM GDP-β-S attenuated inhibition with either peptide. Inhibition was voltage dependent with Som but not with SP. Neurokinin A (1 μM) or B was without effect, implicating NK1 tachykinin receptors. In cell-attached patches with bath-applied drugs, to test for a diffusible messenger, inhibition by SP or Som was only 8%. Thus, SP signaling is voltage independent and PTX insensitive; Som inhibition is voltage dependent and PTX sensitive; and both are membrane delimited.
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U2 - 10.1016/0896-6273(93)90237-L
DO - 10.1016/0896-6273(93)90237-L
M3 - Article
C2 - 7678964
AN - SCOPUS:0027526585
SN - 0896-6273
VL - 10
SP - 11
EP - 20
JO - Neuron
JF - Neuron
IS - 1
ER -