TY - JOUR
T1 - Subcellular location and differential antibody specificity of arginase in tissue culture and whole animals
AU - Spector, Elaine B.
AU - Jenkinson, Christopher P.
AU - Grigor, Murray R.
AU - Kern, Rita M.
AU - Cederbaum, Stephen D.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1994/6
Y1 - 1994/6
N2 - Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
AB - Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
KW - arginase
KW - differential immunoprecipitation
KW - subcellular location mitochondria
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U2 - 10.1016/0736-5748(94)90083-3
DO - 10.1016/0736-5748(94)90083-3
M3 - Article
C2 - 7976488
AN - SCOPUS:0028263957
VL - 12
SP - 337
EP - 342
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
IS - 4
ER -