Subcellular location and differential antibody specificity of arginase in tissue culture and whole animals

Elaine B. Spector, Christopher P. Jenkinson, Murray R. Grigor, Rita M. Kern, Stephen D. Cederbaum

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.

Original languageEnglish (US)
Pages (from-to)337-342
Number of pages6
JournalInternational Journal of Developmental Neuroscience
Volume12
Issue number4
DOIs
StatePublished - Jun 1994

Keywords

  • arginase
  • differential immunoprecipitation
  • subcellular location mitochondria

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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