Subcellular and developmental expression of alternatively spliced forms of fibroblast growth factor 14

Qing Wang, Donald G. McEwen, David M. Ornitz

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Fibroblast growth factors (FGFs) 11-14 comprise a subfamily of FGFs with poorly defined biological function. Here we characterize two isoforms of FGF14 (FGF14-1a and FGF14-1b) that result from the alternative usage of two different first exons. We demonstrate that these isoforms have differential subcellular localization and that they are differentially expressed in various adult tissues. Using in situ hybridization we show that Fgf14 is widely expressed in brain, spinal cord, major arteries and thymus between 12.5 and 14.5 days of mouse embryonic development. We also show that during cerebellar development, Fgf14 is first observed at postnatal day 1 in post mitotic granule cells, and later in development, in migrating and post migratory granule cells. The developmental expression pattern of Fgf14 in the cerebellum is complementary to that of Math1, a marker for proliferating granule cells in the external germinal layer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)283-287
Number of pages5
JournalMechanisms of Development
Volume90
Issue number2
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

    Fingerprint

Keywords

  • Alternative splicing
  • Aorta
  • Central nervous system
  • Cerebellum
  • FGF
  • FGF14
  • FHF4
  • Fibroblast growth factor
  • In situ hybridization
  • Mouse development
  • Nuclear growth factor
  • Nuclear localization
  • Spinal cord
  • Thymus

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this