Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Yuting Li; Francisca M. Acosta; Yumeng Quan; Zhen Li; Sumin Gu; Jean X. Jiang

doi:10.1016/j.xpro.2021.101060

Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Yuting Li, Francisca M. Acosta, Yumeng Quan, Zhen Li, Sumin Gu, Jean X. Jiang

Department of Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).

Original language	English (US)
Article number	101060
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.101060
State	Published - Mar 18 2022

Keywords

Immunology
Microscopy
Model Organisms

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience
General Medicine

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10.1016/j.xpro.2021.101060

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title = "Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model",

abstract = "Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).",

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AU - Li, Yuting

AU - Acosta, Francisca M.

AU - Quan, Yumeng

AU - Li, Zhen

AU - Gu, Sumin

AU - Jiang, Jean X.

PY - 2022/3/18

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AB - Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).

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Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Abstract

Keywords

ASJC Scopus subject areas

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