Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

Yuting Li, Francisca M. Acosta, Yumeng Quan, Zhen Li, Sumin Gu, Jean X. Jiang

Research output: Contribution to journalArticlepeer-review

Abstract

Macrophage (MΦ) activation and promotion of fibrosis are critical processes in lens capsule healing after injury. Here, we detail a protocol that induces MΦ2 formation within the vitreous body of the eye. Our procedure combines the use of an intravitreal injection of a growth factor (CSF-1) and immunofluorescence to confirm the presence of MΦ2 and fibrotic tissue formation. This protocol allows assessment of the distribution of macrophages and quantification of fibrotic tissue formation/sealing within the vitreous body of mouse eyes. For complete details on the use and execution of this profile, please refer to Li et al. (2021), Gerhardt et al. (2003), Kubota et al. (2009).

Original languageEnglish (US)
Article number101060
JournalSTAR Protocols
Volume3
Issue number1
DOIs
StatePublished - Mar 18 2022

Keywords

  • Immunology
  • Microscopy
  • Model Organisms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

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