Studies on the mode of oestrogenic inhibition of hepatic synthesis of α2u globulin and its corresponding messenger ribonucleic acid in rat liver

A. K. Roy, D. J. Dowbenko, M. J. Schiop

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

1. The possible mechanism of the oestrogenic inhibition of the androgen-dependent synthesis of α(2u)-globulin in rat liver was explored by a correlative study of the amounts of α(2u)-globulin, its corresponding mRNA and circulating testosterone in oestrogen-treated male rats. 2. Daily treatments of mature male rats with oestradiol-17β (10 μg/100 g body wt.) decreased and ultimately stopped the hepatic synthesis of α(2u)-globulin as determined by both hepatic and urinary concentrations of the protein. The oestrogen-mediated decrease in the hepatic synthesis of α(2u)-globulin was correlated with a decrease in the mRNA for this protein. 3. Withdrawal of oestrogen resulted in the recovery of α(2u)-globulin synthesis and an increase in mRNA for α(2u)-globulin. 4. At higher doses of oestradiol-17β (50 μg/100 g body wt.), synthesis of α(2u)-globulin was totally suppressed. In addition, this treatment resulted in an extended period of androgen-insensitivity during which treatment with androgens induced synthesis of neither α(2u)-globulin nor its corresponding mRNA. 5. It is concluded that the oestrogenic inhibition of α(2u)-globulin synthesis is mediated by an oestrogen-dependent decrease in the hepatic content of translatable mRNA for α(2u)-globulin.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalBiochemical Journal
Volume164
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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