Studies on the growth of Eikenella corrodens strain 23834

A. Progulske, S. C. Holt

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The growth characteristics and nutritional requirements of Eikenella corrodens strain 23834 were studied. Generation times, growth rates, and cell yields were determined employing a basal medium supplemented with yeast extract (YE), vitamins, amino acids, phosphate, nitrogen, and sulfate. Incubation was in a variety of air, CO2, and N2 atmospheres. Incubation statically in air or in 10% CO2+ 90% air did not affect the growth rate, while Na2CO3, bubbling with various atmospheres, shaking, or anaerobic incubation all decreased the growth rate. Atmospheric CO2 was found not to be a requirement for growth. The addition of 1‐2.5% YE decreased the generation time and increased the cell yield. The growth‐promoting factor(s) of YE was a low molecular weight, water‐soluble, dialyzable substance which is stable to and not extractable by chloroform‐methanol. Vitamins and amino acids singly or in combination were slightly growth‐promoting and purines, pyrimidines, and acetate had no effect on Eikenella growth. Increasing concentrations of phosphate inhibited growth, while nitrate but not sulfate proved essential for growth.

Original languageEnglish (US)
Pages (from-to)2-9
Number of pages8
JournalOral Microbiology and Immunology
Volume2
Issue number1
DOIs
StatePublished - Mar 1987
Externally publishedYes

Keywords

  • Eikenella
  • growth
  • nutritional requirements

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

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