Studies on the differentiation of the retina receptor cell of toad (Bufo raddei Strauch)

S. M. Gu, Y. X. Tong

Research output: Contribution to journalArticle

Abstract

The development of the retinal receptor cell in the young tadpoles (Bufo raddei Strauch), from the stage 20 to the stage 25, was studied by TEM and immunohistochemical method. The morphological differentiation of the photoreceptor cell may be described as follows. The time and the degree of differentiation of the cells in the tadpole retina is asynchronous between central (posterior pole) and peripheral parts of the tadpole retina, namely, they are earlier and higher in the central than in the peripheral. The cells of the outer nuclear layer are undifferentiated at the stage 20. The cells in the posterior part of the retina elongate at the beginning of the stage 21 (Plate I, Fig. 1). This is the first sign of differentiation in the photoreceptor cell. A small hillock-like process forms the inner segment at the scleral pole of the receptor cell. The inner segment is rich in mitochondria, rough-surfaced cytomembrane, free ribosomes, and vesicles. One or two large lipid droplets are also found in the inner segment (Plate I Fig. 2-3). Later on, the connecting structure develops at the tip of the inner segment. The newly formed filaments and the plasma membrane form the outer segment. Its membrane forms some evaginations oriented perpendicularly to the longitudinal axis of the receptor cell. In this way, disks of the outer segment are formed (Plate I Fig. 4-5). The length of the outer segment gradually increases with the number of disks increasing at the base. At the same time, an axon process of receptor cell, extending vitreal, develops synapses with dendrites of the bipolar cell in the outer plexiform layer. At the beginning (the stage 22), the synaptic structure is an immature form that lacks synaptic ribbons and vesicles (Plate II Fig. 8). Later on, ribbons and vesicles are observed in the further developed synaptic structure (Plate II Fig. 9). The toad rhodopsin was prepared by a method of Dewey et al. (1969) and Papermaster & Dreyer (1974) with slight modification. A specific immune serum against the toad rhodopsin was produced in rabbits. Using the indirect Coon's antibody technique, the localization of the rhodopsin antibody and the time when the antibody was seen in the retina of the early developing tadpoles was traced.(ABSTRACT TRUNCATED AT 400 WORDS)

Original languageEnglish (US)
Pages (from-to)27-41
Number of pages15
JournalShi yan sheng wu xue bao = Journal of experimental biology
Volume22
Issue number1
StatePublished - Mar 1 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

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