Studies in the nonhuman in shock: failure to achieve similar responses to infusion of live E. coli organisms and endotoxin

L. B. Hinshaw, J. J. Coalson

Research output: Contribution to journalArticle

Abstract

Concern has often been expressed regarding the relevance of results from animals shocked with endotoxin and the human patient subjected to septic shock. The present study was designed to further test the animal model. Experiments were carried out on young adult baboons, lightly anesthetized, unrestrained, and separately subjected to 1-2 hr infusions of E. coli organisms (aver., 1010 org/kg) or E. coli endotoxin (aver., 25 mg/kg). Animals were found to be significantly more sensitive to E. coli than endotoxin on a canine-dose related basis. Similar responses in the two shock models were hepatic damage, including fibrin thrombi, central lobular congestion and/or hemorrhage, severe glycogen loss, marked mitochondrial swelling, dilatation and fragmentation of rough and smooth endoplasmic reticulum; greatly increased lactate, SGOT, LDH, and F-LDH values; pulmonary perivascular space edema, myocardial mitochondrial edema together with intra- and interfiber edema. The E. coli organism induced shock model was unique in that severe and sustained degrees of systemic hypotension with hypoglycemia and hypoinsulinemia were regularly observed; more notable pulmonary ultrastructural damage was seen; the consistent presence of renal fibrin thrombi was documented; and earlier deaths were recorded with E. coli than in the endotoxin model. Heparin administration (>2000 units/hr) prevented both the development of renal fibrin after E. coli and hepatic fibrin induced by endotoxin. Significant differences in the response to E. coli and endotoxin by the baboon suggest that uniquely complex differences elicited by separate mechanisms are operative in the two forms of shock.

Original languageEnglish (US)
JournalIntensive Care Medicine
Volume3
Issue number3
StatePublished - 1977
Externally publishedYes

Fingerprint

Endotoxins
Shock
Escherichia coli
Fibrin
Edema
Papio
Thrombosis
Mitochondrial Swelling
Kidney
Smooth Endoplasmic Reticulum
Lung
Rough Endoplasmic Reticulum
Liver
Septic Shock
Aspartate Aminotransferases
Glycogen
Hypoglycemia
Hypotension
Heparin
Canidae

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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Studies in the nonhuman in shock : failure to achieve similar responses to infusion of live E. coli organisms and endotoxin. / Hinshaw, L. B.; Coalson, J. J.

In: Intensive Care Medicine, Vol. 3, No. 3, 1977.

Research output: Contribution to journalArticle

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