Structures of the G85R variant of SOD1 in familial amyotrophic lateral sclerosis

Xiaohang Cao, Svetlana V. Antonyuk, Sai V. Seetharaman, Lisa J. Whitson, Alexander B. Taylor, Stephen P. Holloway, Richard W. Strange, Peter A. Doucette, Joan Selverstone Valentine, Ashutosh Tiwari, Lawrence J. Hayward, Shelby Padua, Jeffrey A. Cohlberg, S. Samar Hasnain, P. John Hart

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

Mutations in the gene encoding human copper-zinc superoxide dismutase (SOD1) cause a dominant form of the progressive neurodegenerative disease amyotrophic lateral sclerosis. Transgenic mice expressing the human G85R SOD1 variant develop paralytic symptoms concomitant with the appearance of SOD1-enriched proteinaceous inclusions in their neural tissues. The process(es) through which misfolding or aggregation of G85R SOD1induces motor neuron toxicity is not understood. Here we present structures of the human G85R SOD1 variant determined by single crystal x-ray diffraction. Alterations in structure of the metal-binding loop elements relative to the wild type enzyme suggest a molecular basis for the metal ion deficiency of the G85R SOD1 protein observed in the central nervous system of transgenic mice and in purified recombinant G85R SOD1. These findings support the notion that metal-deficient and/or disulfide-reduced mutant SOD1 species contribute to toxicity in SOD1-linked amyotrophic lateral sclerosis.

Original languageEnglish (US)
Pages (from-to)16169-16177
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number23
DOIs
StatePublished - Jun 6 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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