Structures of Human Pumilio with Noncognate RNAs Reveal Molecular Mechanisms for Binding Promiscuity

Yogesh K. Gupta, Deepak T. Nair, Robin P. Wharton, Aneel K. Aggarwal

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Pumilio is a founder member of the evolutionarily conserved Puf family of RNA-binding proteins that control a number of physiological processes in eukaryotes. A structure of human Pumilio (hPum) Puf domain bound to a Drosophila regulatory sequence showed that each Puf repeat recognizes a single nucleotide. Puf domains in general bind promiscuously to a large set of degenerate sequences, but the structural basis for this promiscuity has been unclear. Here, we describe the structures of hPum Puf domain complexed to two noncognate RNAs, CycBreverse and Puf5. In each complex, one of the nucleotides is ejected from the binding surface, in effect, acting as a "spacer." The complexes also reveal the plasticity of several Puf repeats, which recognize noncanonical nucleotides. Together, these complexes provide a molecular basis for recognition of degenerate binding sites, which significantly increases the number of mRNAs targeted for regulation by Puf proteins in vivo.

Original languageEnglish (US)
Pages (from-to)549-557
Number of pages9
JournalStructure
Volume16
Issue number4
DOIs
StatePublished - Apr 8 2008

Keywords

  • RNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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