Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes

David G. Lowe, Daniel J. Capon, Eric Delwart, Alan Y. Sakaguchi, Susan L. Naylor, David V. Goeddel

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

The human R-ras gene was isolated by low-stringency hybridization with a v-H-ras probe. The predicted 218 amino acid R-ras protein has an amino-terminal extension of 26 residues compared with H-ras p21, and shows 55% amino acid identity; conserved domains include the p21 GTP-binding site and the carboxy-terminal membrane localization sequence. R-ras has at least six exons, with the position of the first intron conserved relative to the Drosophila ras64B and Dictyostelium ras genes; there is no similarity in the exon-intron structure of the R-ras gene and of the mammalian H-, K-, and N-ras proto-oncogenes. Cloned mouse R-ras cDNAs exhibit 88% nucleotide and 94.5% predicted amino acid identity to human R-ras. Human R-ras was localized to chromosome 19, a site different from ras p21 genes. Mouse R-ras is syntenic with c-H-ras on chromosome 7.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalCell
Volume48
Issue number1
DOIs
StatePublished - Jan 16 1987

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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