Structure of the androgen dependent SVS VI protein as derived from cDNA

A. L. Schifman, P. E. Mansson, D. B. Carter, K. Yamada, M. M. Harris, S. E. Harris

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The 11S poly(A +)RNA from rat seminal vesicle was cloned. By hybrid selection of clones reacting to the low molecular weight region of the US peak, a clone containing a new seminal vesicle cDNA sequence called SVS VI was isolated. The DNA sequence of two overlapping cDNAs (pSV24 and pSV33) is presented. The sequence of SVS VI was compared to the previously isolated SVS IV and SVS V cDNAS. Dot hybridization showed that SVS VI is androgen responsive after giving testosterone to castrated rats. The hydrophilicity was analyzed using standard Bionet procedures. All three proteins are extremely variable, rich in α-helix and very water soluble. The computer predicted hydrophilicity is compared for SVS VI, V and IV. A small region in the 3'-non-coding area of SVS VI has high similarity to a region in SVS IV mRNA.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume59
Issue number1-2
DOIs
StatePublished - Sep 1988
Externally publishedYes

Keywords

  • (Rat)
  • Hormone regulation
  • SVS VI, androgen dependent
  • Seminal vesicle
  • mRNA accumulation, comparative

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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