Abstract
The 11S poly(A +)RNA from rat seminal vesicle was cloned. By hybrid selection of clones reacting to the low molecular weight region of the US peak, a clone containing a new seminal vesicle cDNA sequence called SVS VI was isolated. The DNA sequence of two overlapping cDNAs (pSV24 and pSV33) is presented. The sequence of SVS VI was compared to the previously isolated SVS IV and SVS V cDNAS. Dot hybridization showed that SVS VI is androgen responsive after giving testosterone to castrated rats. The hydrophilicity was analyzed using standard Bionet procedures. All three proteins are extremely variable, rich in α-helix and very water soluble. The computer predicted hydrophilicity is compared for SVS VI, V and IV. A small region in the 3'-non-coding area of SVS VI has high similarity to a region in SVS IV mRNA.
Original language | English (US) |
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Pages (from-to) | 57-65 |
Number of pages | 9 |
Journal | Molecular and Cellular Endocrinology |
Volume | 59 |
Issue number | 1-2 |
DOIs | |
State | Published - Sep 1988 |
Externally published | Yes |
Keywords
- (Rat)
- Hormone regulation
- SVS VI, androgen dependent
- Seminal vesicle
- mRNA accumulation, comparative
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology