Structure of Mycobacterium tuberculosis methionine sulfoxide reductase A in complex with protein-bound methionine

Alexander B. Taylor, David M. Benglis, Subramanian Dhandayuthapani, P. John Hart

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Peptide methionine sulfoxide reductase (MsrA) repairs oxidative damage to methionine residues arising from reactive oxygen species and reactive nitrogen intermediates. MsrA activity is found in a wide variety of organisms, and it is implicated as one of the primary defenses against oxidative stress. Disruption of the gene encoding MsrA in several pathogenic bacteria responsible for infections in humans results in the loss of their ability to colonize host cells. Here, we present the X-ray crystal structure of MsrA from the pathogenic bacterium Mycobacterium tuberculosis refined to 1.5 Å resolution. In contrast to the three catalytic cysteine residues found in previously characterized MsrA structures, M. tuberculosis MsrA represents a class containing only two functional cysteine residues. The structure reveals a methionine residue of one MsrA molecule bound at the active site of a neighboring molecule in the crystal lattice and thus serves as an excellent model for protein-bound methionine sulfoxide recognition and repair.

Original languageEnglish (US)
Pages (from-to)4119-4126
Number of pages8
JournalJournal of bacteriology
Volume185
Issue number14
DOIs
StatePublished - Jul 1 2003

    Fingerprint

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this