Structure and functional expression of α1, α2, and β subunits of a novel human neuronal calcium channel subtype

Mark E. Williams, Daniel H. Feldman, Ann F. McCue, Robert Brenner, Gonul Velicelebi, Steven B. Ellis, Michael M. Harpold

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Abstract

The primary structures of human neuronal α1, α2, and β subunits of a voltage-dependent Ca2+ channel were deduced by characterizing cDNAs. The α1 subunit (α1D) directs the recombinant expression of a dihydropyridine-sensitive L-type Ca2+ channel when coexpressed with the β (β2) and the α22b) subunits in Xenopus oocytes. The recombinant channel is also reversibly blocked by 10-15 μM ω-conotoxin. Expression of the α1D subunit alone, or coexpression with the α2b subunit, did not elicit functional Ca2+ channel activity. Thus, the β2 subunit appears to serve an obligatory function, whereas the α2b subunit appears to play an accessory role that potentiates expression of the channel. The primary transcripts encoding the α1D, α2, and β subunits are differentially processed. At least two forms of neuronal α1D were identified. Different forms of α2 and β transcripts were also identified in CNS, skeletal muscle, and aorta tissues.

Original languageEnglish (US)
Pages (from-to)71-84
Number of pages14
JournalNeuron
Volume8
Issue number1
DOIs
StatePublished - Jan 1992

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Williams, M. E., Feldman, D. H., McCue, A. F., Brenner, R., Velicelebi, G., Ellis, S. B., & Harpold, M. M. (1992). Structure and functional expression of α1, α2, and β subunits of a novel human neuronal calcium channel subtype. Neuron, 8(1), 71-84. https://doi.org/10.1016/0896-6273(92)90109-Q