Structure and evolution of opossum, guinea pig, and porcupine cytochrome b genes

D. P. Ma, A. Zharkikh, D. Graur, J. L. VandeBerg, W. H. Li

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

We have sequenced the mitochondrial cytochrome b gene from the guinea pig, the African porcupine, and a South American opossum. A phylogenetic analysis, which includes 22 eutherian and four other vertebrate cytochrome b sequences, indicates that the guinea pig and the porcupine constitute a natural clade (Hystricomorpha) that is not a sister group to the clade of mice and rats (Myomorpha). Therefore, the hypothesis that the Rodentia is paraphyletic receives additional support. The artiodactyls, the perissodactyls, and the cetaceans form a group that is separated from the primates and the rodents. The 26 sequences are used to study the structure/function relationships in cytochrome b, whose function is electron transport. Most of the amino acid residues involved in the two reaction centers are well conserved in evolution. The four histidines that are believed to ligate the two hemes are invariant among the 26 sequences, but their nearby residues are not well conserved in evolution. The eight transmembrane domains represent some of the most divergent regions in the cytochrome b sequence. The rate of nonsynonymous substitution is considerably faster in the human and elephant lineages than in other eutherian lineages; the faster rate might be due to coevolution between cytochrome b and cytochrome c.

Original languageEnglish (US)
Pages (from-to)327-334
Number of pages8
JournalJournal of Molecular Evolution
Volume36
Issue number4
StatePublished - 1993
Externally publishedYes

Fingerprint

Porcupines
Opossums
Cytochromes b
opossums
cytochrome b
pig
guinea pigs
cytochrome
Guinea Pigs
Genes
gene
genes
Rodentia
cytochrome c
Elephantidae
heme
structure-activity relationships
Electron Transport
coevolution
Cytochromes c

Keywords

  • Cavia porcellus
  • Coevolution
  • Cytochrome b
  • Functional constraints
  • Hystrix africaeaustralis
  • Mammalian phylogeny
  • Mitochondrial DNA
  • Monodelphis domestica

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Ma, D. P., Zharkikh, A., Graur, D., VandeBerg, J. L., & Li, W. H. (1993). Structure and evolution of opossum, guinea pig, and porcupine cytochrome b genes. Journal of Molecular Evolution, 36(4), 327-334.

Structure and evolution of opossum, guinea pig, and porcupine cytochrome b genes. / Ma, D. P.; Zharkikh, A.; Graur, D.; VandeBerg, J. L.; Li, W. H.

In: Journal of Molecular Evolution, Vol. 36, No. 4, 1993, p. 327-334.

Research output: Contribution to journalArticle

Ma, DP, Zharkikh, A, Graur, D, VandeBerg, JL & Li, WH 1993, 'Structure and evolution of opossum, guinea pig, and porcupine cytochrome b genes', Journal of Molecular Evolution, vol. 36, no. 4, pp. 327-334.
Ma, D. P. ; Zharkikh, A. ; Graur, D. ; VandeBerg, J. L. ; Li, W. H. / Structure and evolution of opossum, guinea pig, and porcupine cytochrome b genes. In: Journal of Molecular Evolution. 1993 ; Vol. 36, No. 4. pp. 327-334.
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